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Plasmid Files

hmKO

Humanized monomeric Kusabira-Orange fluorescent protein.

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hmKO Sequence and MaphmKO.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (657) BtgI (641) BsaHI (638) TaqII (622) BbsI (610) BmgBI (477) BmrI (432) BanII (431) AleI - MslI (373) BsrFI - SgrAI (370) BglI (338) HaeII (323) EciI (297) PpuMI (281) EcoNI (276) StuI (253) EarI (221) BsaAI - PmlI (186) AflIII (185) AhdI (177) SexAI * (173) StyI (143) MscI (142) Bts α I (124) BaeGI - Bme1580I (91) DrdI (52) BmeT110I (20) AvaI - BsoBI (19) BclI * (11) Start (0) hmKO 657 bp
End  (657)
0 sites
BtgI  (641)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsaHI  (638)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
TaqII  (622)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BbsI  (610)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BmgBI  (477)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BmrI  (432)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
BanII  (431)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
AleI  (373)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (373)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BsrFI  (370)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
SgrAI  (370)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
BglI  (338)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
HaeII  (323)
1 site
R G C G C Y Y C G C G R
EciI  (297)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
PpuMI  (281)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EcoNI  (276)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
StuI  (253)
1 site
A G G C C T T C C G G A
EarI  (221)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BsaAI  (186)
1 site
Y A C G T R R T G C A Y
PmlI  (186)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AflIII  (185)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
AhdI  (177)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SexAI  (173)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
StyI  (143)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (142)
1 site
T G G C C A A C C G G T
BtsαI  (124)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
BaeGI  (91)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (91)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
DrdI  (52)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmeT110I  (20)
1 site
C Y C G R G G R G C Y C
AvaI  (19)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (19)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BclI  (11)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
hmKO
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: humanized monomeric Kusabira-Orange
fluorescent protein
mammalian codon-optimized
hmKO
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: humanized monomeric Kusabira-Orange
fluorescent protein
mammalian codon-optimized
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