Resources
Plasmid Files

hmKeima8.5

Human codon-optimized monomeric red fluorescent protein with a large Stokes shift.

 
To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

 600 400 200 End (666) BsaBI * (640) BtgZI (627) BmgBI (591) BsiEI (579) BglI (535) AcuI (505) BsmI (461) NspI (441) BbsI (412) PasI (411) SacII (333) BtgI (330) BaeGI - Bme1580I (307) ApaLI (303) PluTI (299) SfoI (297) BsaHI - NarI (296) KasI (295) FspI (274) TaqII (258) BmeT110I (254) AvaI - BsoBI (253) Bpu10I (172) StyI (137) BsrBI (47) NmeAIII (41) BsrGI - TatI (38) Start (0) hmKeima8.5 666 bp
End  (666)
0 sites
BsaBI  (640)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtgZI  (627)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BmgBI  (591)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BsiEI  (579)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
BglI  (535)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
AcuI  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsmI  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NspI  (441)
1 site
R C A T G Y Y G T A C R
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PasI  (411)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
SacII  (333)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BtgI  (330)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BaeGI  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (303)
1 site
G T G C A C C A C G T G
PluTI  (299)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (297)
1 site
G G C G C C C C G C G G
BsaHI  (296)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (296)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (295)
1 site
G G C G C C C C G C G G
FspI  (274)
1 site
T G C G C A A C G C G T
TaqII  (258)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BmeT110I  (254)
1 site
C Y C G R G G R G C Y C
AvaI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
Bpu10I  (172)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
StyI  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsrBI  (47)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
NmeAIII  (41)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrGI  (38)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (38)
1 site
W G T A C W W C A T G W
Start  (0)
0 sites
hmKeima8.5
1 .. 666  =  666 bp
222 amino acids  =  25.1 kDa
Product: human codon-optimized monomeric red
fluorescent protein with a large Stokes shift (Guan
et al. 2015)
hmKeima8.5
1 .. 666  =  666 bp
222 amino acids  =  25.1 kDa
Product: human codon-optimized monomeric red
fluorescent protein with a large Stokes shift (Guan
et al. 2015)
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter