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Plasmid Files

iRFP682

Near-infrared fluorescent protein with an emission peak at 682 nm, engineered from a bacterial phytochrome.

 
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 800 600 400 200 End (951) EarI (938) AlwNI * - PflMI * (918) NspI - SphI (792) EcoRV (663) BbvCI - Bpu10I (600) StuI (584) BsgI (571) BspDI * - ClaI * (555) AcuI - Eco57MI (513) SacII (464) BglI (463) BtgI (461) PstI - SbfI (428) SfcI (424) AatII (374) ZraI (372) PspFI (366) BseYI (362) SacI (353) Eco53kI (351) BclI * (330) BtgZI (307) SmaI (249) TspMI - XmaI (247) BbsI (192) BsrBI (185) PluTI (173) SfoI (171) NarI (170) KasI (169) BstXI (85) EcoP15I (63) BfuAI - BspMI (49) BamHI - BstYI (10) Start (0) iRFP682 951 bp
End  (951)
0 sites
EarI  (938)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
AlwNI  (918)
1 site
C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different AlwNI sites may not be compatible.
PflMI  (918)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
NspI  (792)
1 site
R C A T G Y Y G T A C R
SphI  (792)
1 site
G C A T G C C G T A C G
EcoRV  (663)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BbvCI  (600)
1 site
C C T C A G C G G A G T C G
Bpu10I  (600)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
StuI  (584)
1 site
A G G C C T T C C G G A
BsgI  (571)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (555)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (555)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
AcuI  (513)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (513)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
SacII  (464)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BglI  (463)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BtgI  (461)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
PstI  (428)
1 site
C T G C A G G A C G T C
SbfI  (428)
1 site
C C T G C A G G G G A C G T C C
SfcI  (424)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
AatII  (374)
1 site
G A C G T C C T G C A G
ZraI  (372)
1 site
G A C G T C C T G C A G
PspFI  (366)
1 site
C C C A G C G G G T C G
BseYI  (362)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
SacI  (353)
1 site
G A G C T C C T C G A G
Eco53kI  (351)
1 site
G A G C T C C T C G A G
BclI  (330)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BtgZI  (307)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
SmaI  (249)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (247)
1 site
C C C G G G G G G C C C
XmaI  (247)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BbsI  (192)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsrBI  (185)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
PluTI  (173)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (171)
1 site
G G C G C C C C G C G G
NarI  (170)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (169)
1 site
G G C G C C C C G C G G
BstXI  (85)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoP15I  (63)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BfuAI  (49)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (49)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BamHI  (10)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
BstYI  (10)
1 site
R G A T C Y Y C T A G R
Start  (0)
0 sites
iRFP682
1 .. 951  =  951 bp
316 amino acids  =  34.5 kDa
Product: near-infrared fluorescent protein with an
emission peak at 682 nm, engineered from a
bacterial phytochrome (Shcherbakova and
Verkhusha, 2013)
derived from Rhodopseudomonas palustris RpBphP2
iRFP682
1 .. 951  =  951 bp
316 amino acids  =  34.5 kDa
Product: near-infrared fluorescent protein with an
emission peak at 682 nm, engineered from a
bacterial phytochrome (Shcherbakova and
Verkhusha, 2013)
derived from Rhodopseudomonas palustris RpBphP2
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