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Plasmid Files

iRFP702

Near-infrared fluorescent protein with an emission peak at 702 nm, engineered from a bacterial phytochrome.

 
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iRFP702.dna
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800 600 400 200 End (936) BsgI (889) BclI * (821) ApoI (748) AarI - BfuAI - BspMI (728) PluTI (695) SfoI (693) NarI (692) KasI (691) BsiHKAI (680) BaeGI - Bme1580I (674) TatI (611) PaeR7I - SmlI - XhoI (550) DraIII (483) PstI (461) SfcI (457) NmeAIII (450) AcuI - Eco57MI (436) EarI (422) BssHII (400) BspDI * - ClaI * (328) MslI (314) SacII (160) BtgI (157) NaeI (132) NgoMIV (130) BmtI (85) NheI (81) BsmI (59) BsaBI * (46) NruI * (35) AsiSI - PvuI (33) Start (0) iRFP702 936 bp
End  (936)
0 sites
BsgI  (889)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BclI  (821)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
ApoI  (748)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
AarI  (728)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (728)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (728)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PluTI  (695)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (693)
1 site
G G C G C C C C G C G G
NarI  (692)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (691)
1 site
G G C G C C C C G C G G
BsiHKAI  (680)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BaeGI  (674)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (674)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
TatI  (611)
1 site
W G T A C W W C A T G W
PaeR7I  (550)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
SmlI  (550)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
XhoI  (550)
1 site
C T C G A G G A G C T C
DraIII  (483)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PstI  (461)
1 site
C T G C A G G A C G T C
SfcI  (457)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
NmeAIII  (450)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AcuI  (436)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (436)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EarI  (422)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BssHII  (400)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BspDI  (328)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (328)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
MslI  (314)
1 site
C A Y N N N N R T G G T R N N N N Y A C
SacII  (160)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BtgI  (157)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NaeI  (132)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (130)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BmtI  (85)
1 site
G C T A G C C G A T C G
NheI  (81)
1 site
G C T A G C C G A T C G
BsmI  (59)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BsaBI  (46)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
NruI  (35)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
AsiSI  (33)
1 site
G C G A T C G C C G C T A G C G
PvuI  (33)
1 site
C G A T C G G C T A G C
Start  (0)
0 sites
iRFP702
1 .. 936  =  936 bp
311 amino acids  =  34.5 kDa
Product: near-infrared fluorescent protein with an emission peak at 702 nm, engineered from a bacterial phytochrome (Shcherbakova and Verkhusha, 2013)
derived from Rhodopseudomonas palustris RpBphP6
iRFP702
1 .. 936  =  936 bp
311 amino acids  =  34.5 kDa
Product: near-infrared fluorescent protein with an emission peak at 702 nm, engineered from a bacterial phytochrome (Shcherbakova and Verkhusha, 2013)
derived from Rhodopseudomonas palustris RpBphP6
ORF:  1 .. 936  =  936 bp
ORF:  311 amino acids  =  34.5 kDa
ORF:  567 .. 827  =  261 bp
ORF:  86 amino acids  =  9.6 kDa
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