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Plasmid Files

mAmetrine1.1

Yellow fluorescent protein with a large Stokes shift, excitable with violet light. Derived from EGFP. More photostable than the original mAmetrine.

 
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 600 400 200 mAmetrine1.1 End (720) BsrGI (710) Bpu10I (604) BsiHKAI (509) BsiEI (464) EagI (461) NmeAIII (356) EcoO109I - PpuMI (323) SfcI (318) PfoI * (281) EciI (249) Bts α I (214) DrdI (192) BssS α I (181) BtgZI (122) BanI (36) BseRI (31) Start (0) mAmetrine1.1 720 bp
End  (720)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsiHKAI  (509)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BsiEI  (464)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EagI  (461)
1 site
C G G C C G G C C G G C
NmeAIII  (356)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EcoO109I  (323)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (323)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SfcI  (318)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
EciI  (249)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
DrdI  (192)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BssSαI  (181)
1 site
C A C G A G G T G C T C
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Start  (0)
0 sites
mAmetrine1.1
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: photostable yellow fluorescent protein with
a large Stokes shift, excitable with violet light (Ai et
al., 2008)
mAmetrine1.1
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.2 Da
Product: photostable yellow fluorescent protein with
a large Stokes shift, excitable with violet light (Ai et
al., 2008)
mAmetrine1.1
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.6 kDa
Product: photostable yellow fluorescent protein with
a large Stokes shift, excitable with violet light (Ai et
al., 2008)
mAmetrine1.1
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
Product: photostable yellow fluorescent protein with
a large Stokes shift, excitable with violet light (Ai et
al., 2008)
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