mClavGR2

Monomeric photoconvertible green-to-red fluorescent protein derived from Clavularia cyan FP.

Sequence Author: Campbell Lab

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No matches
600 400 200 End (714) TatI - BsrGI (704) BglI (689) BsiHKAI (665) Bpu10I (622) PflMI (600) BmgBI (495) BsaAI (468) EarI (421) BaeGI - Bme1580I (394) BtgZI (315) PvuII (290) SfcI (285) PluTI - HaeII (170) SfoI (168) NarI - BsaHI (167) KasI - BanI (166) FspI (64) BclI * (35) BspHI (26) BsaI (13) Start (0) mClavGR2 mClavGR2 714 bp
End  (714)
0 sites
TatI  (704)
1 site
W G T A C W W C A T G W
BsrGI  (704)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BglI  (689)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsiHKAI  (665)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bpu10I  (622)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PflMI  (600)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BmgBI  (495)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BsaAI  (468)
1 site
Y A C G T R R T G C A Y
EarI  (421)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BaeGI  (394)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (394)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BtgZI  (315)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
PvuII  (290)
1 site
C A G C T G G T C G A C
SfcI  (285)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PluTI  (170)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
HaeII  (170)
1 site
R G C G C Y Y C G C G R
SfoI  (168)
1 site
G G C G C C C C G C G G
NarI  (167)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
BsaHI  (167)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
KasI  (166)
1 site
G G C G C C C C G C G G
BanI  (166)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
FspI  (64)
1 site
T G C G C A A C G C G T
BclI  (35)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspHI  (26)
1 site
T C A T G A A G T A C T
BsaI  (13)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
Start  (0)
0 sites
mClavGR2
1 .. 714  =  714 bp
237 amino acids  =  27.1 kDa
Product: monomeric photoconvertible green-to-red fluorescent protein derived from Clavularia cyan FP (Hoi et al., 2010)
mClavGR2
1 .. 714  =  714 bp
237 amino acids  =  27.1 kDa
Product: monomeric photoconvertible green-to-red fluorescent protein derived from Clavularia cyan FP (Hoi et al., 2010)
ORF:  1 .. 714  =  714 bp
ORF:  237 amino acids  =  27.1 kDa
ORF:  1 .. 714  =  714 bp
ORF:  238 amino acids  =  24.3 kDa  (no start codon)
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Download mClavGR2.dna file

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