mEos4b

Fixation-resistant truly monomeric green-to-red photoswitchable fluorescent protein.
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No matches
600 400 200 End (684) NspI - SphI (643) PflMI (576) PluTI - HaeII (569) SfoI (567) NarI * - BsaHI (566) KasI (565) Bpu10I (539) AcuI - Eco57MI (504) BanII (425) KpnI (371) Acc65I (367) MslI (325) BsrDI (312) BstBI (287) HindIII (276) Bsu36I (254) BsmI (181) BtsI - BtsαI (176) EcoRV (168) EciI (158) MflI * - BstYI (123) BtgI - StyI - NcoI (119) BaeGI - Bme1580I (65) Start (0) mEos4b mEos4b 684 bp
End  (684)
0 sites
NspI  (643)
1 site
R C A T G Y Y G T A C R
SphI  (643)
1 site
G C A T G C C G T A C G
PflMI  (576)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
PluTI  (569)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
HaeII  (569)
1 site
R G C G C Y Y C G C G R
SfoI  (567)
1 site
G G C G C C C C G C G G
NarI  (566)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
BsaHI  (566)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
KasI  (565)
1 site
G G C G C C C C G C G G
Bpu10I  (539)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AcuI  (504)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (504)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BanII  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
KpnI  (371)
1 site
G G T A C C C C A T G G
Acc65I  (367)
1 site
G G T A C C C C A T G G
MslI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BsrDI  (312)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BstBI  (287)
1 site
T T C G A A A A G C T T
HindIII  (276)
1 site
A A G C T T T T C G A A
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsmI  (181)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BtsI  (176)
1 site
G C A G T G N N C G T C A C
BtsαI  (176)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
EcoRV  (168)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
EciI  (158)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
MflI  (123)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (123)
1 site
R G A T C Y Y C T A G R
BtgI  (119)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
StyI  (119)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NcoI  (119)
1 site
C C A T G G G G T A C C
BaeGI  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
Start  (0)
0 sites
mEos4b
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: fixation-resistant truly monomeric green-to-red photoswitchable fluorescent protein (Paez-Segala et al., 2015)
derived from mEos2
mEos4b
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: fixation-resistant truly monomeric green-to-red photoswitchable fluorescent protein (Paez-Segala et al., 2015)
derived from mEos2
ORF:  1 .. 684  =  684 bp
ORF:  227 amino acids  =  25.9 kDa
ORF:  1 .. 507  =  507 bp
ORF:  169 amino acids  =  19.4 kDa
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Download mEos4b.dna file

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