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Plasmid Files

mIFP

Naturally monomeric infrared fluorescent protein derived from a truncated bacteriophytochrome.

 
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 800 600 400 200 End (960) BpmI - Eco57MI (952) NruI (925) BsaBI * (919) BtgZI (906) HaeII (898) AfeI (896) NspI (768) SfcI (676) BsaWI (666) MslI (632) AccI (592) BsaHI (589) BstEII (574) ApoI (530) EarI (509) NaeI (389) BsrFI - NgoMIV (387) BsrDI (380) BtgI (284) BsgI (238) AlwNI - BstAPI (231) BciVI (109) BanII - Bsp1286I (82) AflIII - MluI (35) BsmI (25) BbvCI - Bpu10I (21) KpnI (10) Acc65I - BanI (6) Start (0) mIFP 960 bp
End  (960)
0 sites
BpmI  (952)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (952)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NruI  (925)
1 site
T C G C G A A G C G C T
BsaBI  (919)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtgZI  (906)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
HaeII  (898)
1 site
R G C G C Y Y C G C G R
AfeI  (896)
1 site
A G C G C T T C G C G A
NspI  (768)
1 site
R C A T G Y Y G T A C R
SfcI  (676)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
BsaWI  (666)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
MslI  (632)
1 site
C A Y N N N N R T G G T R N N N N Y A C
AccI  (592)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaHI  (589)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
BstEII  (574)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
ApoI  (530)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EarI  (509)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
NaeI  (389)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
BsrFI  (387)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
NgoMIV  (387)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
BsrDI  (380)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BtgI  (284)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsgI  (238)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (231)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BstAPI  (231)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BciVI  (109)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BanII  (82)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (82)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
AflIII  (35)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
MluI  (35)
1 site
A C G C G T T G C G C A
BsmI  (25)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BbvCI  (21)
1 site
C C T C A G C G G A G T C G
Bpu10I  (21)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
KpnI  (10)
1 site
G G T A C C C C A T G G
Acc65I  (6)
1 site
G G T A C C C C A T G G
BanI  (6)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
Start  (0)
0 sites
mIFP
1 .. 960  =  960 bp
320 amino acids  =  35.1 kDa
Product: naturally monomeric infrared fluorescent
protein derived from a truncated
bacteriophytochrome (Yu et al., 2015)
mIFP
1 .. 960  =  960 bp
320 amino acids  =  35.1 kDa
Product: naturally monomeric infrared fluorescent
protein derived from a truncated
bacteriophytochrome (Yu et al., 2015)
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