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Plasmid Files

mKO

Monomeric Kusabira-Orange fluorescent protein.

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mKO Sequence and MapmKO.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (657) BtgZI (574) BmrI (380) HincII - HpaI (378) ApoI (364) FspI - FspAI (323) BstBI (293) MmeI - StuI (266) EarI (221) BsaAI - PmlI (186) AhdI (177) StyI (143) MscI (142) MluI (129) MfeI (74) DrdI (52) Start (0) mKO 657 bp
End  (657)
0 sites
BtgZI  (574)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BmrI  (380)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
HincII  (378)
1 site
G T Y R A C C A R Y T G
HpaI  (378)
1 site
G T T A A C C A A T T G
ApoI  (364)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
FspI  (323)
1 site
T G C G C A A C G C G T
FspAI  (323)
1 site
R T G C G C A Y Y A C G C G T R
BstBI  (293)
1 site
T T C G A A A A G C T T
MmeI  (266)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
StuI  (266)
1 site
A G G C C T T C C G G A
EarI  (221)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BsaAI  (186)
1 site
Y A C G T R R T G C A Y
PmlI  (186)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (177)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
StyI  (143)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (142)
1 site
T G G C C A A C C G G T
MluI  (129)
1 site
A C G C G T T G C G C A
MfeI  (74)
1 site
C A A T T G G T T A A C
DrdI  (52)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
Start  (0)
0 sites
mKO
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: monomeric Kusabira-Orange fluorescent
protein (Karasawa et al., 2004)
mKO
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: monomeric Kusabira-Orange fluorescent
protein (Karasawa et al., 2004)
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