mKO2

Monomeric Kusabira-Orange 2 fluorescent protein.

Sequence Author: MBL International

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No matches
600 400 200 End (657) BfuAI - BspMI (618) BtgZI (574) BsaI (566) BbsI (535) BmrI (380) HpaI - HincII (378) ApoI (364) FspI - FspAI (323) BstBI (293) StuI - MmeI (266) EarI (221) PmlI - BsaAI (186) AhdI (177) NmeAIII (167) MluI (129) MfeI (74) DrdI (52) Start (0) mKO2 mKO2 657 bp
End  (657)
0 sites
BfuAI  (618)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (618)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BtgZI  (574)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaI  (566)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BbsI  (535)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BmrI  (380)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
HpaI  (378)
1 site
G T T A A C C A A T T G
HincII  (378)
1 site
G T Y R A C C A R Y T G
ApoI  (364)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
FspI  (323)
1 site
T G C G C A A C G C G T
FspAI  (323)
1 site
R T G C G C A Y Y A C G C G T R
BstBI  (293)
1 site
T T C G A A A A G C T T
StuI  (266)
1 site
A G G C C T T C C G G A
MmeI  (266)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EarI  (221)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
PmlI  (186)
1 site
C A C G T G G T G C A C
BsaAI  (186)
1 site
Y A C G T R R T G C A Y
AhdI  (177)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NmeAIII  (167)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MluI  (129)
1 site
A C G C G T T G C G C A
MfeI  (74)
1 site
C A A T T G G T T A A C
DrdI  (52)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
Start  (0)
0 sites
mKO2
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: monomeric Kusabira-Orange 2 fluorescent protein
mKO2
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: monomeric Kusabira-Orange 2 fluorescent protein
ORF:  1 .. 657  =  657 bp
ORF:  218 amino acids  =  24.5 kDa
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Download mKO2.dna file

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Individual Sequences & Maps

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