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Plasmid Files

mKeima-Red

Monomeric Keima-Red fluorescent protein.

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mKeima-Red Sequence and MapmKeima-Red.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (669) HaeII - PluTI (567) SfoI (565) BsaHI - NarI (564) BanI - KasI (563) DraI - SwaI (522) ApoI (517) MmeI (470) BsmI (461) BsmBI (442) BsgI (317) TatI (304) BseRI (288) Bsu36I (254) BstXI (161) BtgZI (98) Start (0) mKeima-Red 669 bp
End  (669)
0 sites
HaeII  (567)
1 site
R G C G C Y Y C G C G R
PluTI  (567)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (565)
1 site
G G C G C C C C G C G G
BsaHI  (564)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (564)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
BanI  (563)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
KasI  (563)
1 site
G G C G C C C C G C G G
DraI  (522)
1 site
T T T A A A A A A T T T
SwaI  (522)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
ApoI  (517)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
MmeI  (470)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsmI  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BsmBI  (442)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsgI  (317)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
TatI  (304)
1 site
W G T A C W W C A T G W
BseRI  (288)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BstXI  (161)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BtgZI  (98)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
Start  (0)
0 sites
mKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
Product: monomeric Keima-Red fluorescent protein
mKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
Product: monomeric Keima-Red fluorescent protein
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