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Plasmid Files

mKikGR1

Monomeric photoconvertible Kikume green-to-red fluorescent protein.

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mKikGR1 Sequence and MapmKikGR1.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (702) HaeII - PluTI (680) SfoI (678) BsaHI - NarI (677) BanI - KasI (676) SfcI (669) EcoO109I - PpuMI (665) PfoI (661) Bpu10I (650) NspI (649) BsaAI - DraIII (582) MscI (512) EaeI (510) Bsu36I (254) AccI (230) BsmI (181) EcoRV (168) EciI (158) EarI (155) DrdI (130) BaeGI - Bme1580I - Bsp1286I (65) Start (0) mKikGR1 702 bp
End  (702)
0 sites
HaeII  (680)
1 site
R G C G C Y Y C G C G R
PluTI  (680)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (678)
1 site
G G C G C C C C G C G G
BsaHI  (677)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (677)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
BanI  (676)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
KasI  (676)
1 site
G G C G C C C C G C G G
SfcI  (669)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
EcoO109I  (665)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (665)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
PfoI  (661)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
Bpu10I  (650)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NspI  (649)
1 site
R C A T G Y Y G T A C R
BsaAI  (582)
1 site
Y A C G T R R T G C A Y
DraIII  (582)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
MscI  (512)
1 site
T G G C C A A C C G G T
EaeI  (510)
1 site
Y G G C C R R C C G G Y
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AccI  (230)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsmI  (181)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
EcoRV  (168)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
EciI  (158)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
EarI  (155)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
DrdI  (130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BaeGI  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
Bsp1286I  (65)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
Start  (0)
0 sites
mKikGR1
1 .. 702  =  702 bp
233 amino acids  =  26.6 kDa
Product: monomeric photoconvertible Kikume
green-to-red fluorescent protein
mKikGR1
1 .. 702  =  702 bp
233 amino acids  =  26.6 kDa
Product: monomeric photoconvertible Kikume
green-to-red fluorescent protein
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