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Plasmid Files

mMaroon1

Bright, rapidly maturing, monomeric far-red fluorescent protein.

 
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mMaroon1.dna
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600 400 200 End (735) BsrGI - TatI (725) AlwNI (674) MscI (667) EaeI (665) DraIII (657) PshAI (640) BsaHI (584) SmlI (571) BpuEI (556) BstYI (547) PstI (529) AarI - BfuAI - BspMI (527) SfcI (525) AcuI (517) BsgI (505) ApaI - BanII (495) PspOMI (491) BglI (476) MspA1I (460) PspFI (432) BseYI (428) MmeI (419) NmeAIII (405) PfoI * (341) BpmI (325) BbsI (308) Bsu36I (266) TsoI (238) TaqII (139) BstBI (83) NspI (39) BseRI (31) BclI * (23) Start (0) mMaroon1 735 bp
End  (735)
0 sites
BsrGI  (725)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (725)
1 site
W G T A C W W C A T G W
AlwNI  (674)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
MscI  (667)
1 site
T G G C C A A C C G G T
EaeI  (665)
1 site
Y G G C C R R C C G G Y
DraIII  (657)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PshAI  (640)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BsaHI  (584)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
SmlI  (571)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (556)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstYI  (547)
1 site
R G A T C Y Y C T A G R
PstI  (529)
1 site
C T G C A G G A C G T C
AarI  (527)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (527)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (527)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SfcI  (525)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AcuI  (517)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsgI  (505)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaI  (495)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (495)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (491)
1 site
G G G C C C C C C G G G
BglI  (476)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
MspA1I  (460)
1 site
C M G C K G G K C G M C
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
MmeI  (419)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (405)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (341)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BpmI  (325)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BbsI  (308)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
TsoI  (238)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
TaqII  (139)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BstBI  (83)
1 site
T T C G A A A A G C T T
NspI  (39)
1 site
R C A T G Y Y G T A C R
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BclI  (23)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
mMaroon1
1 .. 735  =  735 bp
244 amino acids  =  27.6 kDa
Product: bright, rapidly maturing, monomeric far-red fluorescent protein (Bajar et al., 2016)
mMaroon1
1 .. 735  =  735 bp
244 amino acids  =  27.6 kDa
Product: bright, rapidly maturing, monomeric far-red fluorescent protein (Bajar et al., 2016)
ORF:  1 .. 735  =  735 bp
ORF:  244 amino acids  =  27.6 kDa
ORF:  1 .. 735  =  735 bp
ORF:  245 amino acids  =  25.4 kDa  (no start codon)
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