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Plasmid Files

mMiCy1

Monomeric Midoriishi-Cyan fluorescent protein.

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mMiCy1 Sequence and MapmMiCy1.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 mMiCy1 End (699) HpaI (678) BbvCI - Bpu10I (665) TaqII (628) StyI (618) BmeT110I (580) AvaI - BsoBI (579) BsrDI (578) BstEII (520) PspFI (438) BseYI (434) MfeI (428) BpmI - Eco57MI (354) BsmBI (349) PvuII (332) BseRI (329) SfcI (323) NdeI (285) AhdI (189) Bsu36I (155) BclI * (143) AatII (133) ZraI (131) BsaHI (130) EcoNI (116) BsiEI - PvuI (89) Bts α I (69) BtgZI (9) Start (0) mMiCy1 699 bp
End  (699)
0 sites
HpaI  (678)
1 site
G T T A A C C A A T T G
BbvCI  (665)
1 site
C C T C A G C G G A G T C G
Bpu10I  (665)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
TaqII  (628)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
StyI  (618)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BmeT110I  (580)
1 site
C Y C G R G G R G C Y C
AvaI  (579)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (579)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BsrDI  (578)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BstEII  (520)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspFI  (438)
1 site
C C C A G C G G G T C G
BseYI  (434)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
MfeI  (428)
1 site
C A A T T G G T T A A C
BpmI  (354)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (354)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsmBI  (349)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PvuII  (332)
1 site
C A G C T G G T C G A C
BseRI  (329)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
SfcI  (323)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
NdeI  (285)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
AhdI  (189)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
Bsu36I  (155)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BclI  (143)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AatII  (133)
1 site
G A C G T C C T G C A G
ZraI  (131)
1 site
G A C G T C C T G C A G
BsaHI  (130)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
EcoNI  (116)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsiEI  (89)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
PvuI  (89)
1 site
C G A T C G G C T A G C
BtsαI  (69)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
BtgZI  (9)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
Start  (0)
0 sites
mMiCy1
1 .. 699  =  699 bp
232 amino acids  =  26.0 kDa
Product: monomeric Midoriishi-Cyan fluorescent
protein
mMiCy1
1 .. 699  =  699 bp
232 amino acids  =  26.0 kDa
Product: monomeric Midoriishi-Cyan fluorescent
protein
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