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Plasmid Files

mNeptune2.5

Brighter version of the far-red fluorescent protein mNeptune2 with slightly blue-shifted spectra.

 
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 600 400 200 End (735) BmrI (703) AlwNI (674) DraIII (657) PshAI (640) AccI (587) BsaHI (584) SmlI (571) BpuEI (556) BstYI (547) Bts α I (522) BsgI (505) BglI (476) MspA1I (460) PspFI (432) BseYI (428) MmeI (419) NmeAIII (405) PfoI * (341) BpmI (325) Bsu36I (266) PasI (231) BsmI (172) EcoO109I (157) BsiHKAI (91) ApaLI (87) XcmI (51) NspI (39) BseRI (31) BclI * (23) Start (0) mNeptune2.5 735 bp
End  (735)
0 sites
BmrI  (703)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
AlwNI  (674)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
DraIII  (657)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PshAI  (640)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
AccI  (587)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaHI  (584)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
SmlI  (571)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (556)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstYI  (547)
1 site
R G A T C Y Y C T A G R
BtsαI  (522)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
BsgI  (505)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (476)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
MspA1I  (460)
1 site
C M G C K G G K C G M C
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
MmeI  (419)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (405)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (341)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BpmI  (325)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Bsu36I  (266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PasI  (231)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BsmI  (172)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
EcoO109I  (157)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsiHKAI  (91)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (87)
1 site
G T G C A C C A C G T G
XcmI  (51)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
NspI  (39)
1 site
R C A T G Y Y G T A C R
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BclI  (23)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
mNeptune2.5
1 .. 735  =  735 bp
244 amino acids  =  27.5 kDa
Product: brighter version of the far-red fluorescent
protein mNeptune2 with slightly blue-shifted spectra
mNeptune2.5
1 .. 735  =  735 bp
244 amino acids  =  27.5 kDa
Product: brighter version of the far-red fluorescent
protein mNeptune2 with slightly blue-shifted spectra
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