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Plasmid Files

mPapaya1

Monomeric yellow fluorescent protein derived from the Zoanthus fluorescent protein zFP538

 
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 600 400 200 mPapaya1 End (714) BsrGI - TatI (704) NspI (682) Bsu36I (628) BglI (603) BaeGI - Bme1580I - Bsp1286I (587) BsrDI (546) BsiEI (540) PflMI (446) AlwNI (355) BsaAI (290) BsaWI (221) BstEII (217) DraI (210) BpmI (125) BseRI (58) Start (0) mPapaya1 714 bp
End  (714)
0 sites
BsrGI  (704)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (704)
1 site
W G T A C W W C A T G W
NspI  (682)
1 site
R C A T G Y Y G T A C R
Bsu36I  (628)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BglI  (603)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BaeGI  (587)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (587)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
Bsp1286I  (587)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BsrDI  (546)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BsiEI  (540)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
PflMI  (446)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AlwNI  (355)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BsaAI  (290)
1 site
Y A C G T R R T G C A Y
BsaWI  (221)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BstEII  (217)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
DraI  (210)
1 site
T T T A A A A A A T T T
BpmI  (125)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BseRI  (58)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Start  (0)
0 sites
mPapaya1
1 .. 714  =  714 bp
237 amino acids  =  26.9 kDa
Product: monomeric yellow fluorescent protein
derived from the Zoanthus fluorescent protein
zFP538
mammalian codon-optimized
mPapaya1
1 .. 714  =  714 bp
237 amino acids  =  26.9 kDa
Product: monomeric yellow fluorescent protein
derived from the Zoanthus fluorescent protein
zFP538
mammalian codon-optimized
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