mPlum

Far-red monomeric derivative of DsRed fluorescent protein.

Sequence Author: Clontech (TaKaRa)

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No matches
600 400 200 End (681) BsrFI - SgrAI (670) BssHII (648) AleI (631) TaqII (601) BsgI (583) PvuII - MspA1I (567) MscI (550) BstXI (546) DrdI (529) BbvCI - Bpu10I (496) BsrBI (447) PspFI (432) PflMI - BseYI (428) BbsI (424) BtgI - NcoI (422) MmeI (419) PstI - SbfI (344) SfcI (340) BsaAI (219) AhdI (183) BaeGI - Bme1580I (124) BssSI - BssSαI (76) BanII (64) BsiHKAI (53) Start (0) mPlum mPlum 681 bp
End  (681)
0 sites
BsrFI  (670)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (670)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BssHII  (648)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
AleI  (631)
1 site
C A C N N N N G T G G T G N N N N C A C
TaqII  (601)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BsgI  (583)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuII  (567)
1 site
C A G C T G G T C G A C
MspA1I  (567)
1 site
C M G C K G G K C G M C
MscI  (550)
1 site
T G G C C A A C C G G T
BstXI  (546)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
DrdI  (529)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BbvCI  (496)
1 site
C C T C A G C G G A G T C G
Bpu10I  (496)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsrBI  (447)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
PspFI  (432)
1 site
C C C A G C G G G T C G
PflMI  (428)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgI  (422)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (422)
1 site
C C A T G G G G T A C C
MmeI  (419)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PstI  (344)
1 site
C T G C A G G A C G T C
SbfI  (344)
1 site
C C T G C A G G G G A C G T C C
SfcI  (340)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BsaAI  (219)
1 site
Y A C G T R R T G C A Y
AhdI  (183)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BaeGI  (124)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (124)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BssSI  (76)
1 site
C A C G A G G T G C T C
BssSαI  (76)
1 site
C A C G A G G T G C T C
BanII  (64)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (53)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Start  (0)
0 sites
mPlum
1 .. 681  =  681 bp
226 amino acids  =  25.6 kDa
Product: far-red monomeric derivative of DsRed fluorescent protein (Wang et al., 2004)
mammalian codon-optimized
mPlum
1 .. 681  =  681 bp
226 amino acids  =  25.6 kDa
Product: far-red monomeric derivative of DsRed fluorescent protein (Wang et al., 2004)
mammalian codon-optimized
ORF:  1 .. 681  =  681 bp
ORF:  226 amino acids  =  25.6 kDa
ORF:  3 .. 680  =  678 bp
ORF:  226 amino acids  =  25.4 kDa  (no start codon)
ORF:  1 .. 681  =  681 bp
ORF:  227 amino acids  =  22.3 kDa  (no start codon)
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