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Plasmid Files

mRuby3

Monomeric red fluorescent protein derived from mRuby2, with improved brightness and photostability.

 
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 600 400 200 End (711) BsrGI - TatI (704) BsaAI - SnaBI (642) BseRI (634) MslI (574) Bts α I (522) MscI (515) BsgI (505) EcoP15I (488) BtgZI (474) BbsI (424) BpuEI (361) AccI (359) BanII - BsiHKAI - Bsp1286I - SacI (356) Eco53kI (354) SmlI (340) Bsu36I (266) DraI (252) BsiEI (233) EagI (230) BmgBI (191) EcoO109I - PpuMI (157) BsiWI (113) HincII (69) TaqII (54) BspQI - EarI - SapI (11) Start (0) mRuby3 711 bp
End  (711)
0 sites
BsrGI  (704)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (704)
1 site
W G T A C W W C A T G W
BsaAI  (642)
1 site
Y A C G T R R T G C A Y
SnaBI  (642)
1 site
T A C G T A A T G C A T
BseRI  (634)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
MslI  (574)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BtsαI  (522)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
MscI  (515)
1 site
T G G C C A A C C G G T
BsgI  (505)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EcoP15I  (488)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BtgZI  (474)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpuEI  (361)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AccI  (359)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BanII  (356)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (356)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (356)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
SacI  (356)
1 site
G A G C T C C T C G A G
Eco53kI  (354)
1 site
G A G C T C C T C G A G
SmlI  (340)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
Bsu36I  (266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
DraI  (252)
1 site
T T T A A A A A A T T T
BsiEI  (233)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EagI  (230)
1 site
C G G C C G G C C G G C
BmgBI  (191)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
EcoO109I  (157)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (157)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BsiWI  (113)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
HincII  (69)
1 site
G T Y R A C C A R Y T G
TaqII  (54)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BspQI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (11)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
Start  (0)
0 sites
mRuby3
1 .. 711  =  711 bp
237 amino acids  =  26.6 kDa
Product: monomeric red fluorescent protein derived
from mRuby2, with improved brightness and
photostability (Bajar et al., 2016)
mRuby3
1 .. 711  =  711 bp
237 amino acids  =  26.6 kDa
Product: monomeric red fluorescent protein derived
from mRuby2, with improved brightness and
photostability (Bajar et al., 2016)
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