mScarlet-H

Monomeric red fluorescent protein evolved from a synthetic template. More photostable than mScarlet.
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No matches
600 400 200 End TatI - BsrGI XcmI BsrFI - SgrAI AleI MslI HincII AccI SalI BsgI MmeI EciI NotI EagI BstAPI BsrBI PspFI BseYI BbsI SacII MspA1I EcoO109I * AhdI StyI BstEII BssSI - BssSαI BanII BsiHKAI ApaLI BtsI - BtsαI BclI * Start mScarlet-H mScarlet-H 696 bp
End  (696)
0 sites
TatI  (689)
1 site
W G T A C W W C A T G W
BsrGI  (689)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
XcmI  (675)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrFI  (670)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (670)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AleI  (631)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (631)
1 site
C A Y N N N N R T G G T R N N N N Y A C
HincII  (588)
1 site
G T Y R A C C A R Y T G
AccI  (587)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (586)
1 site
G T C G A C C A G C T G
BsgI  (583)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MmeI  (578)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EciI  (541)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
NotI  (513)
1 site
G C G G C C G C C G C C G G C G
EagI  (513)
1 site
C G G C C G G C C G G C
BstAPI  (495)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BsrBI  (447)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
SacII  (378)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
MspA1I  (377)
1 site
C M G C K G G K C G M C
EcoO109I  (212)
1 site
R G G N C C Y Y C C N G G R
* Blocked by Dcm methylation.
Sticky ends from different EcoO109I sites may not be compatible.
AhdI  (183)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
StyI  (149)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BstEII  (144)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BssSI  (76)
1 site
C A C G A G G T G C T C
BssSαI  (76)
1 site
C A C G A G G T G C T C
BanII  (64)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (53)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (49)
1 site
G T G C A C C A C G T G
BtsI  (26)
1 site
G C A G T G N N C G T C A C
BtsαI  (26)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BclI  (23)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
mScarlet-H
1 .. 696  =  696 bp
232 amino acids  =  26.4 kDa
Product: monomeric red fluorescent protein evolved from a synthetic template (Bindels et al., 2016)
more photostable than mScarlet
mScarlet-H
1 .. 696  =  696 bp
232 amino acids  =  26.4 kDa
Product: monomeric red fluorescent protein evolved from a synthetic template (Bindels et al., 2016)
more photostable than mScarlet
ORF:  1 .. 696  =  696 bp
ORF:  232 amino acids  =  26.4 kDa
ORF:  3 .. 254  =  252 bp
ORF:  83 amino acids  =  9.0 kDa  (no start codon)
ORF:  1 .. 696  =  696 bp
ORF:  232 amino acids  =  23.2 kDa  (no start codon)
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Download mScarlet-H.dna file

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