mWasabi

Green variant of the teal fluorescent protein mTFP1.
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No matches
600 400 200 End (711) TatI - BsrGI (701) XcmI (687) BstEII (645) MflI * - BstYI (614) DraIII (597) BglI (568) SfcI (555) HincII (540) AatII (497) ZraI (495) BsaAI (468) PspFI (444) PflMI - BseYI (440) BsrFI (436) SmlI (385) BpuEI (370) MmeI (365) StyI - NcoI (350) MslI (349) AvaI - BsoBI (277) EcoO109I (224) BanII - Bsp1286I (171) BstXI (150) BanI (133) MscI (86) Start (0) mWasabi mWasabi 711 bp
End  (711)
0 sites
TatI  (701)
1 site
W G T A C W W C A T G W
BsrGI  (701)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
XcmI  (687)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BstEII  (645)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
MflI  (614)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (614)
1 site
R G A T C Y Y C T A G R
DraIII  (597)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BglI  (568)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfcI  (555)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
HincII  (540)
1 site
G T Y R A C C A R Y T G
AatII  (497)
1 site
G A C G T C C T G C A G
ZraI  (495)
1 site
G A C G T C C T G C A G
BsaAI  (468)
1 site
Y A C G T R R T G C A Y
PspFI  (444)
1 site
C C C A G C G G G T C G
PflMI  (440)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BseYI  (440)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BsrFI  (436)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
SmlI  (385)
1 site
C T Y R A G G A R Y T C

Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (370)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MmeI  (365)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
StyI  (350)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NcoI  (350)
1 site
C C A T G G G G T A C C
MslI  (349)
1 site
C A Y N N N N R T G G T R N N N N Y A C
AvaI  (277)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (277)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
EcoO109I  (224)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BanII  (171)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (171)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BstXI  (150)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BanI  (133)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
MscI  (86)
1 site
T G G C C A A C C G G T
Start  (0)
0 sites
mWasabi
1 .. 711  =  711 bp
236 amino acids  =  26.9 kDa
Product: green variant of teal fluorescent protein mTFP1 (Ai et al., 2008)
mWasabi
1 .. 711  =  711 bp
236 amino acids  =  26.9 kDa
Product: green variant of teal fluorescent protein mTFP1 (Ai et al., 2008)
ORF:  1 .. 711  =  711 bp
ORF:  236 amino acids  =  26.9 kDa
ORF:  3 .. 542  =  540 bp
ORF:  179 amino acids  =  20.3 kDa  (no start codon)
ORF:  1 .. 711  =  711 bp
ORF:  237 amino acids  =  23.9 kDa  (no start codon)
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