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Plasmid Files

pAcGFP1-C1

Vector for fusing AcGFP1 to the N-terminus of a partner protein. For other reading frames, use pAcGFP1‑C2 or pAcGFP1‑C3.

To see this sequence with restriction sites, features, and translations, please download
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pAcGFP1-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4673) ApaLI (4359) BsaI (3744) RsrII (3271) BsrDI (2988) PflFI - Tth111I (2873) FspI (2857) EagI (2661) BspDI * - ClaI * (2595) StuI (2576) BseRI (2573) SfiI (2530) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) BstEII (795) Bpu10I (805) BssHII (939) EcoNI (1237) PpuMI (1242) PflMI (1311) BspEI (1330) BglII (1339) PaeR7I - XhoI (1343) Eco53kI (1348) SacI (1350) HindIII (1352) EcoRI (1359) PstI (1368) SalI (1369) AccI (1370) Acc65I (1375) KpnI (1379) SacII (1382) PspOMI (1383) TspMI - XmaI (1386) ApaI (1387) SmaI (1388) BamHI (1390) XbaI * (1402) BclI * (1412) MfeI (1505) HpaI (1518) MluI (1641) SexAI * (2344) pAcGFP1-C1 4731 bp
PciI  (4673)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4359)
1 site
G T G C A C C A C G T G
BsaI  (3744)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (3271)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2988)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2873)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2873)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2857)
1 site
T G C G C A A C G C G T
EagI  (2661)
1 site
C G G C C G G C C G G C
BspDI  (2595)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2595)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2576)
1 site
A G G C C T T C C G G A
BseRI  (2573)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SfiI  (2530)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
BstEII  (795)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
Bpu10I  (805)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BssHII  (939)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoNI  (1237)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PpuMI  (1242)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
PflMI  (1311)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BspEI  (1330)
1 site
T C C G G A A G G C C T
BglII  (1339)
1 site
A G A T C T T C T A G A
PaeR7I  (1343)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1343)
1 site
C T C G A G G A G C T C
Eco53kI  (1348)
1 site
G A G C T C C T C G A G
SacI  (1350)
1 site
G A G C T C C T C G A G
HindIII  (1352)
1 site
A A G C T T T T C G A A
EcoRI  (1359)
1 site
G A A T T C C T T A A G
PstI  (1368)
1 site
C T G C A G G A C G T C
SalI  (1369)
1 site
G T C G A C C A G C T G
AccI  (1370)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1375)
1 site
G G T A C C C C A T G G
KpnI  (1379)
1 site
G G T A C C C C A T G G
SacII  (1382)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (1383)
1 site
G G G C C C C C C G G G
TspMI  (1386)
1 site
C C C G G G G G G C C C
XmaI  (1386)
1 site
C C C G G G G G G C C