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Plasmid Files

pAmCyan1-C1

Vector for fusing AmCyan1 to the N-terminus of a partner protein.

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pAmCyan1-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4643) ApaLI (4329) BsaI (3714) PfoI (3500) RsrII (3241) BsrDI (2958) PflFI - Tth111I (2843) FspI (2827) PluTI (2728) SfoI (2726) NarI (2725) KasI (2724) EagI (2631) BspDI * - ClaI * (2565) BseRI (2543) SfiI (2500) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) BstEII (753) BbsI (1039) KflI - PpuMI (1047) BmgBI (1098) BstXI - PmlI (1197) EcoNI (1231) BsgI (1270) AleI (1285) BspEI (1300) BglII (1309) PaeR7I - XhoI (1313) Eco53kI (1318) SacI (1320) HindIII (1322) EcoRI (1329) SalI (1339) AccI (1340) Acc65I (1345) KpnI (1349) SacII (1352) PspOMI (1353) TspMI - XmaI (1356) ApaI (1357) SmaI (1358) BamHI (1360) XbaI * (1372) BclI * (1382) MfeI (1475) HpaI (1488) Bts α I (1564) MluI (1611) SexAI * (2314) pAmCyan1-C1 4701 bp
PciI  (4643)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4329)
1 site
G T G C A C C A C G T G
BsaI  (3714)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3500)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3241)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2958)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2843)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2843)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2827)
1 site
T G C G C A A C G C G T
PluTI  (2728)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2726)
1 site
G G C G C C C C G C G G
NarI  (2725)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2724)
1 site
G G C G C C C C G C G G
EagI  (2631)
1 site
C G G C C G G C C G G C
BspDI  (2565)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2565)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
BseRI  (2543)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SfiI  (2500)
1 site