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Plasmid Files

pDsRed-Express2-C1

Vector for fusing rapidly maturing and noncytotoxic DsRed-Express2 to the N-terminus of a partner protein.

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pDsRed-Express2-C1 Sequence and MappDsRed-Express2-C1.dna
Map and Sequence File   
Sequence Author:  Clontech
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 PciI (4622) EcoO109I (3802) BsaI (3693) PfoI (3479) RsrII (3220) BsrDI (2937) PflFI - Tth111I (2822) FspI (2806) PluTI (2707) SfoI (2705) NarI (2704) KasI (2703) EagI (2610) BspDI * - ClaI * (2544) StuI (2525) SfiI (2479) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) AarI (741) AhdI (792) SbfI (953) PmlI (975) BbsI (1033) PflMI * (1131) AleI (1240) BssHII (1257) BglII (1288) PaeR7I - XhoI (1292) Eco53kI (1297) SacI (1299) HindIII (1301) EcoRI (1308) SalI (1318) AccI (1319) Acc65I (1324) KpnI (1328) SacII (1331) PspOMI (1332) TspMI - XmaI (1335) ApaI (1336) SmaI (1337) BamHI (1339) XbaI * (1351) stop codons BclI * (1361) MfeI (1454) HpaI (1467) Bts α I (1543) MluI (1590) DraIII (1820) pDsRed-Express2-C1 4680 bp
PciI  (4622)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3802)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3693)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3479)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3220)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2937)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2822)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2822)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2806)
1 site
T G C G C A A C G C G T
PluTI  (2707)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (2705)
1 site
G G C G C C C C G C G G
NarI  (2704)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (2703)
1 site
G G C G C C C C G C G G
EagI  (2610)
1 site
C G G C C G G C C G G C
BspDI  (2544)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2544)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2525)
1 site
A G G C C T T C C G G A
SfiI  (2479)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
AarI  (741)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
AhdI  (792)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (953)
1 site
C C T G C A G G G G A C G T C C
PmlI  (975)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BbsI  (1033)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PflMI  (1131)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
AleI  (1240)
1 site
C A C N N N N G T G G T G N N N N C A C
BssHII  (1257)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BglII  (1288)
1 site
A G A T C T T C T A G A
PaeR7I  (1292)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1292)
1 site
C T C G A G G A G C T C
Eco53kI  (1297)
1 site
G A G C T C C T C G A G
SacI  (1299)
1 site
G A G C T C C T C G A G
HindIII  (1301)
1 site
A A G C T T T T C G A A
EcoRI  (1308)
1 site
G A A T T C C T T A A G
SalI  (1318)
1 site
G T C G A C C A G C T G
AccI  (1319)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1324)
1 site
G G T A C C C C A T G G
KpnI  (1328)
1 site
G G T A C C C C A T G G
SacII  (1331)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PspOMI  (1332)
1 site
G G G C C C C C C G G G
TspMI  (1335)
1 site
C C C G G G G G G C C C
XmaI  (1335)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (1336)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (1337)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1339)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1351)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (1361)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1454)
1 site
C A A T T G G T T A A C
HpaI  (1467)
1 site
G T T A A C C A A T T G
BtsαI  (1543)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
MluI  (1590)
1 site
A C G C G T T G C G C A
DraIII  (1820)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NeoR/KanR
2576 .. 3370  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
2576 .. 3370  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
DsRed-Express2
613 .. 1287  =  675 bp
225 amino acids  =  25.7 kDa
Product: noncytotoxic tetrameric variant of DsRed
fluorescent protein
mammalian codon-optimized
DsRed-Express2
613 .. 1287  =  675 bp
225 amino acids  =  25.7 kDa
Product: noncytotoxic tetrameric variant of DsRed
fluorescent protein
mammalian codon-optimized
ori
3978 .. 4566  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3978 .. 4566  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
1596 .. 2051  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
1596 .. 2051  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
2184 .. 2541  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2184 .. 2541  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
1468 .. 1589  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1468 .. 1589  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2078 .. 2182  =  105 bp
AmpR promoter
2078 .. 2182  =  105 bp
MCS
1288 .. 1344  =  57 bp
multiple cloning site of fluorescent protein plasmids
MCS
1288 .. 1344  =  57 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3602 .. 3649  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3602 .. 3649  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
stop codons
1353 .. 1363  =  11 bp
stop codons in all three reading frames
stop codons
1353 .. 1363  =  11 bp
stop codons in all three reading frames
SV40 ori
2392 .. 2527  =  136 bp
SV40 origin of replication
SV40 ori
2392 .. 2527  =  136 bp
SV40 origin of replication
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