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Plasmid Files

pECFP-C1

Vector for fusing ECFP to the N-terminus of a partner protein

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pECFP-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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AflIII - PciI (4673) ApaLI (4359) EcoO109I (3853) BsaI (3744) RsrII (3271) BsrDI (2988) PflFI - Tth111I (2873) FspI (2857) MscI (2837) PluTI (2758) SfoI (2756) NarI (2755) KasI (2754) EagI (2661) BspDI * - ClaI * (2595) StuI (2576) SfiI (2530) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) BsrGI (1322) BspEI (1330) BglII (1339) PaeR7I - XhoI (1343) Eco53kI (1348) SacI (1350) HindIII (1352) EcoRI (1359) PstI (1368) SalI (1369) AccI (1370) Acc65I (1375) KpnI (1379) SacII (1382) PspOMI (1383) TspMI - XmaI (1386) ApaI (1387) SmaI (1388) BamHI (1390) XbaI * (1402) BclI * (1412) MfeI (1505) HpaI (1518) AflII (1637) DraIII (1871) SexAI * (2344) pECFP-C1 4731 bp
AflIII  (4673)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4673)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4359)
1 site
G T G C A C C A C G T G
EcoO109I  (3853)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3744)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (3271)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2988)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2873)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2873)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2857)
1 site
T G C G C A A C G C G T
MscI  (2837)
1 site
T G G C C A A C C G G T
PluTI  (2758)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2756)
1 site
G G C G C C C C G C G G
NarI  (2755)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2754)
1 site
G G C G C C C C G C G G
EagI  (2661)
1 site
C G G C C G G C C G G C
BspDI  (2595)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2595)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2576)
1 site
A G G C C T T C C G G A
SfiI  (2530)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A