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Plasmid Files

pEGFP-C3

Vector for fusing EGFP to the N-terminus of a partner protein. For other reading frames, use pEGFP‑C1 or pEGFP‑C2.

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pEGFP-C3.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4669) ApaLI (4355) EcoO109I (3849) BsaI (3740) RsrII (3267) BsrDI (2984) PflFI - Tth111I (2869) FspI (2853) MscI (2833) PluTI (2754) SfoI (2752) NarI (2751) KasI (2750) EagI (2657) BspDI * - ClaI * (2591) StuI (2572) SfiI (2526) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) BsrGI (1322) ScaI (1330) BglII (1335) PaeR7I - XhoI (1339) Eco53kI (1344) SacI (1346) HindIII (1348) EcoRI (1355) PstI (1364) SalI (1365) AccI (1366) Acc65I (1371) KpnI (1375) SacII (1378) PspOMI (1379) TspMI - XmaI (1382) ApaI (1383) SmaI (1384) BamHI (1386) XbaI * (1398) BclI * (1408) MfeI (1501) HpaI (1514) MluI (1637) DraIII (1867) SexAI * (2340) pEGFP-C3 4727 bp
PciI  (4669)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4355)
1 site
G T G C A C C A C G T G
EcoO109I  (3849)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3740)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (3267)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2984)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2869)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2869)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2853)
1 site
T G C G C A A C G C G T
MscI  (2833)
1 site
T G G C C A A C C G G T
PluTI  (2754)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2752)
1 site
G G C G C C C C G C G G
NarI  (2751)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2750)
1 site
G G C G C C C C G C G G
EagI  (2657)
1 site
C G G C C G G C C G G C
BspDI  (2591)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2591)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2572)
1 site
A G G C C T T C C G G A
SfiI  (2526)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
BsrGI  (1322)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
ScaI  (1330)
1 site
A G T A C T T C A T G A
BglII  (1335)
1 site
A G A T C T T C T A G A
PaeR7I  (1339)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1339)
1 site