Sticky ends from different EcoO109I sites may not be compatible.
RsrII (3262) 1 site
CGGWCCGGCCWGGC
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
BsrDI (2979) 1 site
GCAATGNNCGTTAC
Sticky ends from different BsrDI sites may not be compatible.
PflFI (2864) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible.
Tth111I (2864) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible.
FspI (2848) 1 site
TGCGCAACGCGT
PluTI (2749) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI (2747) 1 site
GGCGCCCCGCGG
NarI (2746) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI (2745) 1 site
GGCGCCCCGCGG
EagI (2652) 1 site
CGGCCGGCCGGC
BspDI (2586) 1 site
ATCGATTAGCTA
* Blocked by Dam methylation.
ClaI (2586) 1 site
ATCGATTAGCTA
* Blocked by Dam methylation.
SfiI (2521) 1 site
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI (7) 1 site
ATTAATTAATTA
NdeI (234) 1 site
CATATGGTATAC
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI (340) 1 site
TACGTAATGCAT
NheI (591) 1 site
GCTAGCCGATCG
BmtI (595) 1 site
GCTAGCCGATCG
AfeI (596) 1 site
AGCGCTTCGCGA
AgeI (600) 1 site
ACCGGTTGGCCA
AleI (613) 1 site
CACNNNNGTGGTGNNNNCAC
BsrGI (653) 1 site
TGTACAACATGT
BsrGI is typically used at 37°C, but is even more active at 60°C.
BbsI (911) 1 site
GAAGACNNCTTCTGNN(N)4
Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C.
BsmBI (1042) 1 site
CGTCTCNGCAGAGN(N)4
Sticky ends from different BsmBI sites may not be compatible.
PflMI (1209) 1 site
CCANNNNNTGGGGTNNNNNACC
Sticky ends from different PflMI sites may not be compatible.
PshAI (1243) 1 site
GACNNNNGTCCTGNNNNCAG
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.