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Plasmid Files

pPA-TagRFP-C

Vector for fusing photoactivatable TagRFP to the N-terminus of a partner protein.

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pPA-TagRFP-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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PciI (4667) EcoO109I (3847) RsrII (3265) BsrDI (2982) PflFI - Tth111I (2867) FspI (2851) PluTI (2752) SfoI (2750) NarI (2749) KasI (2748) EagI (2655) BspDI * - ClaI * (2589) StuI (2570) SfiI (2524) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) BsrGI (656) BbsI (914) BspEI (1324) BglII (1333) PaeR7I - XhoI (1337) Eco53kI (1342) SacI (1344) HindIII (1346) EcoRI (1353) PstI (1362) Acc65I (1369) KpnI (1373) SacII (1376) PspOMI (1377) TspMI - XmaI (1380) ApaI (1381) SmaI (1382) BamHI (1384) XbaI * (1396) BclI * (1406) MfeI (1499) HpaI (1512) Bts α I (1588) MluI (1635) SexAI * (2338) pPA-TagRFP-C 4725 bp
PciI  (4667)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3847)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
RsrII  (3265)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2982)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2851)
1 site
T G C G C A A C G C G T
PluTI  (2752)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2750)
1 site
G G C G C C C C G C G G
NarI  (2749)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2748)
1 site
G G C G C C C C G C G G
EagI  (2655)
1 site
C G G C C G G C C G G C
BspDI  (2589)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2589)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2570)
1 site
A G G C C T T C C G G A
SfiI  (2524)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
BsrGI  (656)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BbsI  (914)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BspEI  (1324)
1 site
T C C G G A A G G C C T
BglII  (1333)
1 site
A G A T C T T C T A G A
PaeR7I  (1337)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1337)
1 site
C T C G A G G A G C T C
Eco53kI  (1342)
1 site
G A G C T C C T C G A G
SacI  (1344)
1 site
G A G C T C C T C G A G
HindIII  (1346)
1 site
A A G C T T T T C G A A
EcoRI  (1353)
1 site
G A A T T C C T T A A G
PstI  (1362)
1 site
C T G C A G G A C G T C
Acc65I  (1369)
1 site
G G T A C C C C A T G G
KpnI  (1373)
1 site
G G T A C C C C A T G G
SacII  (1376)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (1377)
1 site
G G G C C C C C C G G G
TspMI  (1380)
1 site
C C C G G G G G G C C C
XmaI  (1380)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (1381)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (1382)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1384)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1396)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (1406)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1499)
1 site
C A A T T G G T T A A C
HpaI  (1512)
1 site
G T T A A C C A A T T G
BtsαI  (1588)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
MluI  (1635)
1 site
A C G C G T T G C G C A
SexAI  (2338)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NeoR/KanR
2621 .. 3415  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2621 .. 3415  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
PA-TagRFP
607 .. 1311  =  705 bp
235 amino acids  =  26.5 kDa
Product: photoactivatable variant of the monomeric red fluorescent protein TagRFP
mammalian codon-optimized
PA-TagRFP
607 .. 1311  =  705 bp
235 amino acids  =  26.5 kDa
Product: photoactivatable variant of the monomeric red fluorescent protein TagRFP
mammalian codon-optimized
ori
4023 .. 4611  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4023 .. 4611  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1641 .. 2096  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1641 .. 2096  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
2229 .. 2586  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2229 .. 2586  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1513 .. 1634  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1513 .. 1634  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2123 .. 2227  =  105 bp
AmpR promoter
2123 .. 2227  =  105 bp
MCS
1324 .. 1389  =  66 bp
multiple cloning site of fluorescent protein plasmids
MCS
1324 .. 1389  =  66 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3647 .. 3694  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3647 .. 3694  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
SV40 ori
2437 .. 2572  =  136 bp
SV40 origin of replication
SV40 ori
2437 .. 2572  =  136 bp
SV40 origin of replication
ORF:  607 .. 1404  =  798 bp
ORF:  265 amino acids  =  29.2 kDa
ORF:  3436 .. 3885  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  2621 .. 3415  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2793 .. 3179  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  592 .. 1422  =  831 bp
ORF:  276 amino acids  =  28.8 kDa
ORF:  720 .. 965  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  2930 .. 3466  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3641 .. 3874  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
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