Resources
Plasmid Files

pPA-TagRFP-C

Vector for fusing photoactivatable TagRFP to the N-terminus of a partner protein.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pPA-TagRFP-C Sequence and MappPA-TagRFP-C.dna
Map and Sequence File   
Sequence Author:  Evrogen
Download Free Trial Get SnapGene Viewer

 PciI (4667) EcoO109I (3847) RsrII (3265) BsrDI (2982) PflFI - Tth111I (2867) FspI (2851) PluTI (2752) SfoI (2750) NarI (2749) KasI (2748) EagI (2655) BspDI * - ClaI * (2589) StuI (2570) SfiI (2524) SV40 promoter AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) BsrGI (656) BbsI (914) BspEI (1324) BglII (1333) PaeR7I - XhoI (1337) Eco53kI (1342) SacI (1344) HindIII (1346) EcoRI (1353) PstI (1362) Acc65I (1369) KpnI (1373) SacII (1376) PspOMI (1377) TspMI - XmaI (1380) ApaI (1381) SmaI (1382) BamHI (1384) XbaI * (1396) BclI * (1406) MfeI (1499) HpaI (1512) Bts α I (1588) MluI (1635) SexAI * (2338) pPA-TagRFP-C 4725 bp
PciI  (4667)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3847)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
RsrII  (3265)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2982)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2851)
1 site
T G C G C A A C G C G T
PluTI  (2752)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (2750)
1 site
G G C G C C C C G C G G
NarI  (2749)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (2748)
1 site
G G C G C C C C G C G G
EagI  (2655)
1 site
C G G C C G G C C G G C
BspDI  (2589)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2589)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2570)
1 site
A G G C C T T C C G G A
SfiI  (2524)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
BsrGI  (656)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BbsI  (914)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BspEI  (1324)
1 site
T C C G G A A G G C C T
BglII  (1333)
1 site
A G A T C T T C T A G A
PaeR7I  (1337)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1337)
1 site
C T C G A G G A G C T C
Eco53kI  (1342)
1 site
G A G C T C C T C G A G
SacI  (1344)
1 site
G A G C T C C T C G A G
HindIII  (1346)
1 site
A A G C T T T T C G A A
EcoRI  (1353)
1 site
G A A T T C C T T A A G
PstI  (1362)
1 site
C T G C A G G A C G T C
Acc65I  (1369)
1 site
G G T A C C C C A T G G
KpnI  (1373)
1 site
G G T A C C C C A T G G
SacII  (1376)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PspOMI  (1377)
1 site
G G G C C C C C C G G G
TspMI  (1380)
1 site
C C C G G G G G G C C C
XmaI  (1380)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (1381)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (1382)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1384)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1396)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (1406)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1499)
1 site
C A A T T G G T T A A C
HpaI  (1512)
1 site
G T T A A C C A A T T G
BtsαI  (1588)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
MluI  (1635)
1 site
A C G C G T T G C G C A
SexAI  (2338)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NeoR/KanR
2621 .. 3415  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
2621 .. 3415  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
PA-TagRFP
607 .. 1311  =  705 bp
235 amino acids  =  26.5 kDa
Product: photoactivatable variant of the monomeric
red fluorescent protein TagRFP
mammalian codon-optimized
PA-TagRFP
607 .. 1311  =  705 bp
235 amino acids  =  26.5 kDa
Product: photoactivatable variant of the monomeric
red fluorescent protein TagRFP
mammalian codon-optimized
ori
4023 .. 4611  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4023 .. 4611  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
1641 .. 2096  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
1641 .. 2096  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
2229 .. 2586  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2229 .. 2586  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
1513 .. 1634  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1513 .. 1634  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2123 .. 2227  =  105 bp
AmpR promoter
2123 .. 2227  =  105 bp
MCS
1324 .. 1389  =  66 bp
multiple cloning site of fluorescent protein plasmids
MCS
1324 .. 1389  =  66 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3647 .. 3694  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3647 .. 3694  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
SV40 ori
2437 .. 2572  =  136 bp
SV40 origin of replication
SV40 ori
2437 .. 2572  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter