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Plasmid Files

pTurboFP602-N

Vector for fusing TurboFP602 to the C-terminus of a partner protein.

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pTurboFP602-N.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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AflIII - PciI (4663) EcoO109I (3843) PfoI (3520) RsrII (3261) BsrDI (2978) PflFI - Tth111I (2863) FspI (2847) PluTI (2748) SfoI (2746) NarI (2745) KasI (2744) BspDI * - ClaI * (2585) StuI (2566) BseRI (2563) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) HindIII (622) EcoRI (629) PstI (638) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) AleI (679) BstXI (681) BclI * (701) BsrGI (728) BbsI (986) BsgI (1183) PshAI (1318) ScaI (1351) NotI (1389) XbaI * (1399) MfeI (1495) HpaI (1508) AflII (1627) SexAI * (2334) pTurboFP602-N 4721 bp
AflIII  (4663)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4663)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3843)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (3520)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3261)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2978)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2863)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2863)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2847)
1 site
T G C G C A A C G C G T
PluTI  (2748)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2746)
1 site
G G C G C C C C G C G G
NarI  (2745)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2744)
1 site
G G C G C C C C G C G G
BspDI  (2585)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2585)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2566)
1 site
A G G C C T T C C G G A
BseRI  (2563)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
BglII  (609)
1 site
A G A T C T T C T A G A
PaeR7I  (613)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (613)
1 site
C T C G A G G A G C T C
Eco53kI  (618)
1 site
G A G C T C C T C G A G
SacI  (620)
1 site
G A G C T C C T C G A G
HindIII  (622)
1 site
A A G C T T T T C G