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Plasmid Files

pTurboFP602-PRL

Promoterless TurboFP602 reporter vector.

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pTurboFP602-PRL Sequence and MappTurboFP602-PRL.dna
Map and Sequence File   
Sequence Author:  Evrogen
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 AfeI (14) AflIII - PciI (4081) EcoO109I (3261) PfoI (2938) RsrII (2679) BsrDI (2396) PflFI - Tth111I (2281) FspI (2265) PluTI (2166) SfoI (2164) NarI (2163) KasI (2162) BglII (27) PaeR7I - XhoI (31) Eco53kI (36) SacI (38) HindIII (40) EcoRI (47) PstI (56) SalI (57) AccI (58) Acc65I (63) KpnI (67) SacII (70) PspOMI (71) TspMI - XmaI (74) ApaI (75) SmaI (76) BamHI (78) AgeI (84) AleI (97) BstXI (99) BclI * (119) BsrGI (146) BbsI (404) BsgI (601) PshAI (736) ScaI (769) NotI (807) XbaI * (817) MfeI (913) HpaI (926) AflII (1045) SexAI * (1752) BseRI (1981) StuI (1984) BspDI * - ClaI * (2003) pTurboFP602-PRL 4139 bp
AfeI  (14)
1 site
A G C G C T T C G C G A
AflIII  (4081)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4081)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3261)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (2938)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2679)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2396)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2281)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2281)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2265)
1 site
T G C G C A A C G C G T
PluTI  (2166)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (2164)
1 site
G G C G C C C C G C G G
NarI  (2163)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (2162)
1 site
G G C G C C C C G C G G
BglII  (27)
1 site
A G A T C T T C T A G A
PaeR7I  (31)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (31)
1 site
C T C G A G G A G C T C
Eco53kI  (36)
1 site
G A G C T C C T C G A G
SacI  (38)
1 site
G A G C T C C T C G A G
HindIII  (40)
1 site
A A G C T T T T C G A A
EcoRI  (47)
1 site
G A A T T C C T T A A G
PstI  (56)
1 site
C T G C A G G A C G T C
SalI  (57)
1 site
G T C G A C C A G C T G
AccI  (58)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (63)
1 site
G G T A C C C C A T G G
KpnI  (67)
1 site
G G T A C C C C A T G G
SacII  (70)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PspOMI  (71)
1 site
G G G C C C C C C G G G
TspMI  (74)
1 site
C C C G G G G G G C C C
XmaI  (74)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (75)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (76)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (78)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
AgeI  (84)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
AleI  (97)
1 site
C A C N N N N G T G G T G N N N N C A C
BstXI  (99)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BclI  (119)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsrGI  (146)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BbsI  (404)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsgI  (601)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PshAI  (736)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
ScaI  (769)
1 site
A G T A C T T C A T G A
NotI  (807)
1 site
G C G G C C G C C G C C G G C G
XbaI  (817)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (913)
1 site
C A A T T G G T T A A C
HpaI  (926)
1 site
G T T A A C C A A T T G
AflII  (1045)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
SexAI  (1752)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BseRI  (1981)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
StuI  (1984)
1 site
A G G C C T T C C G G A
BspDI  (2003)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2003)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
NeoR/KanR
2035 .. 2829  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
2035 .. 2829  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
TurboFP602
97 .. 804  =  708 bp
235 amino acids  =  26.3 kDa
Product: red-shifted derivative of red fluorescent
protein from Entacmaea quadricolor
mammalian codon-optimized
TurboFP602
97 .. 804  =  708 bp
235 amino acids  =  26.3 kDa
Product: red-shifted derivative of red fluorescent
protein from Entacmaea quadricolor
mammalian codon-optimized
ori
3437 .. 4025  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3437 .. 4025  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
1055 .. 1510  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
1055 .. 1510  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
1643 .. 2000  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
1643 .. 2000  =  358 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
927 .. 1048  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
927 .. 1048  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1537 .. 1641  =  105 bp
AmpR promoter
1537 .. 1641  =  105 bp
MCS
12 .. 89  =  78 bp
multiple cloning site of fluorescent protein plasmids
MCS
12 .. 89  =  78 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3061 .. 3108  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3061 .. 3108  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
SV40 ori
1851 .. 1986  =  136 bp
SV40 origin of replication
SV40 ori
1851 .. 1986  =  136 bp
SV40 origin of replication
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