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Plasmid Files

pTurboGFP-B

Vector for expressing TurboGFP in bacteria.

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pTurboGFP-B.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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BbsI (4089) AatII (4033) ZraI (4031) PvuI (3481) FspI (3333) AseI (3283) NmeAIII (3259) BglI (3231) BsaI (3172) AhdI (3111) AlwNI (2634) AflIII - PciI (2218) BstAPI (2042) NdeI (2041) PaeR7I - XhoI (1) PsiI (49) MfeI (59) BamHI (127) KasI (277) NarI (278) SfoI (279) PluTI (281) NgoMIV (469) EagI - NaeI (471) FseI (473) AarI (581) PstI (707) PspOMI (714) ApaI - BanII (718) HindIII (828) NheI (948) BmtI (952) Bpu10I (973) PvuII (1100) BspEI (1196) PasI (1432) MscI (1467) XbaI (1805) PfoI (1862) PflFI - Tth111I (1965) AccI (1990) BstZ17I (1991) pTurboGFP-B 4103 bp
BbsI  (4089)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AatII  (4033)
1 site
G A C G T C C T G C A G
ZraI  (4031)
1 site
G A C G T C C T G C A G
PvuI  (3481)
1 site
C G A T C G G C T A G C
FspI  (3333)
1 site
T G C G C A A C G C G T
AseI  (3283)
1 site
A T T A A T T A A T T A
NmeAIII  (3259)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (3231)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (3172)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3111)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2634)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2218)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2218)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstAPI  (2042)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
NdeI  (2041)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
BamHI  (127)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KasI  (277)
1 site
G G C G C C C C G C G G
NarI  (278)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (279)
1 site
G G C G C C C C G C G G
PluTI  (281)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
NgoMIV  (469)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
EagI  (471)
1 site
C G G C C G G C C G G C
NaeI  (471)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
FseI  (473)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
AarI  (581)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
PstI  (707)
1 site
C T G C A G G A C G T C
PspOMI  (714)
1 site
G G G C C C C C C G G G
ApaI  (718)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (718)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
HindIII  (828)
1 site
A A G C T T T T C G A A
NheI  (948)
1 site
G C T A G C C G A T C G
BmtI  (952)
1 site
G C T A G C C G A T C G
Bpu10I  (973)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu