pTurboYFP-PRL

Promoterless TurboYFP reporter vector.

Sequence Author: Evrogen

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No matches
AfeI (14) AflIII - PciI (4105) EcoO109I (3285) PfoI (2962) RsrII (2703) PflFI - Tth111I (2305) FspI (2289) BglII (27) PaeR7I - XhoI (31) Eco53kI (36) SacI (38) HindIII (40) EcoRI (47) PstI (56) SalI (57) AccI (58) Acc65I (63) KpnI (67) PspOMI (71) TspMI - XmaI (74) ApaI (75) SmaI (76) BamHI (78) AgeI (84) SgrAI (244) AleI (274) XmnI (334) PflMI (498) BlpI (715) BssHII (768) BspEI (811) NotI (831) XbaI * (841) MfeI (937) HpaI (950) AflII (1069) DraIII (1303) CsiI - SexAI * (1776) BglI - SfiI (1962) BseRI (2005) StuI (2008) BspDI * - ClaI * (2027) pTurboYFP-PRL 4163 bp
AfeI  (14)
1 site
A G C G C T T C G C G A
AflIII  (4105)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4105)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3285)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (2962)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2703)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (2305)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2305)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2289)
1 site
T G C G C A A C G C G T
BglII  (27)
1 site
A G A T C T T C T A G A
PaeR7I  (31)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (31)
1 site
C T C G A G G A G C T C
Eco53kI  (36)
1 site
G A G C T C C T C G A G
SacI  (38)
1 site
G A G C T C C T C G A G
HindIII  (40)
1 site
A A G C T T T T C G A A
EcoRI  (47)
1 site
G A A T T C C T T A A G
PstI  (56)
1 site
C T G C A G G A C G T C
SalI  (57)
1 site
G T C G A C C A G C T G
AccI  (58)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (63)
1 site
G G T A C C C C A T G G
KpnI  (67)
1 site
G G T A C C C C A T G G
PspOMI  (71)
1 site
G G G C C C C C C G G G
TspMI  (74)
1 site
C C C G G G G G G C C C
XmaI  (74)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
ApaI  (75)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (76)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (78)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AgeI  (84)
1 site
A C C G G T T G G C C A
SgrAI  (244)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AleI  (274)
1 site
C A C N N N N G T G G T G N N N N C A C
XmnI  (334)
1 site
G A A N N N N T T C C T T N N N N A A G
PflMI  (498)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BlpI  (715)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BssHII  (768)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BspEI  (811)
1 site
T C C G G A A G G C C T
NotI  (831)
1 site
G C G G C C G C C G C C G G C G
XbaI  (841)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (937)
1 site
C A A T T G G T T A A C
HpaI  (950)
1 site
G T T A A C C A A T T G
AflII  (1069)
1 site
C T T A A G G A A T T C
DraIII  (1303)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
CsiI  (1776)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (1776)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BglI  (1962)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (1962)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (2005)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI  (2008)
1 site
A G G C C T T C C G G A
BspDI  (2027)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2027)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
NeoR/KanR
2059 .. 2853  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2059 .. 2853  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
TurboYFP
97 .. 828  =  732 bp
243 amino acids  =  26.9 kDa
Product: enhanced derivative of PhiYFP
mammalian codon-optimized
TurboYFP
97 .. 828  =  732 bp
243 amino acids  =  26.9 kDa
Product: enhanced derivative of PhiYFP
mammalian codon-optimized
ori
3461 .. 4049  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3461 .. 4049  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1079 .. 1534  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1079 .. 1534  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
1667 .. 2024  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
1667 .. 2024  =  358 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
951 .. 1072  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
951 .. 1072  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1561 .. 1665  =  105 bp
AmpR promoter
1561 .. 1665  =  105 bp
MCS
12 .. 89  =  78 bp
multiple cloning site of fluorescent protein plasmids
MCS
12 .. 89  =  78 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3085 .. 3132  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3085 .. 3132  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
SV40 ori
1875 .. 2010  =  136 bp
SV40 origin of replication
SV40 ori
1875 .. 2010  =  136 bp
SV40 origin of replication
ORF:  97 .. 828  =  732 bp
ORF:  243 amino acids  =  26.9 kDa
ORF:  2059 .. 2853  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2231 .. 2617  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  2874 .. 3323  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  2368 .. 2904  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3079 .. 3312  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
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Individual Sequences & Maps

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