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Plasmid Files

pZsGreen1-1

Promoterless ZsGreen1 reporter vector.

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pZsGreen1-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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AflIII - PciI (4068) ApaLI (3754) EcoO109I (3248) BsaI (3139) PfoI (2925) RsrII (2666) BsrDI (2383) FspI (2252) MscI (2232) AfeI (14) BglII (27) PaeR7I - XhoI (31) Eco53kI (36) SacI (38) HindIII (40) EcoRI (47) PstI (56) Acc65I (63) KpnI (67) SacII (70) PspOMI (71) TspMI - XmaI (74) ApaI (75) SmaI (76) BamHI (78) AgeI (84) BclI * (179) SgrAI (184) AhdI (276) BmgBI (588) BsrGI - TatI (647) Bpu10I (789) NotI (794) XbaI * (804) MfeI (900) HpaI (913) Bts α I (989) AflII (1032) DraIII (1266) SexAI * (1739) SfiI (1925) StuI (1971) BspDI * - ClaI * (1990) pZsGreen1-1 4126 bp
AflIII  (4068)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4068)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (3754)
1 site
G T G C A C C A C G T G
EcoO109I  (3248)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3139)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (2925)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2666)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2383)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
FspI  (2252)
1 site
T G C G C A A C G C G T
MscI  (2232)
1 site
T G G C C A A C C G G T
AfeI  (14)
1 site
A G C G C T T C G C G A
BglII  (27)
1 site
A G A T C T T C T A G A
PaeR7I  (31)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (31)
1 site
C T C G A G G A G C T C
Eco53kI  (36)
1 site
G A G C T C C T C G A G
SacI  (38)
1 site
G A G C T C C T C G A G
HindIII  (40)
1 site
A A G C T T T T C G A A
EcoRI  (47)
1 site
G A A T T C C T T A A G
PstI  (56)
1 site
C T G C A G G A C G T C
Acc65I  (63)
1 site
G G T A C C C C A T G G
KpnI  (67)
1 site
G G T A C C C C A T G G
SacII  (70)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (71)
1 site
G G G C C C C C C G G G
TspMI  (74)
1 site
C C C G G G G G G C C C
XmaI  (74)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (75)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (76)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (78)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AgeI  (84)
1 site
A C C G G T T G G C C A
BclI  (179)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SgrAI  (184)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AhdI  (276)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmgBI  (588)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BsrGI  (647)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (647)
1 site
W G T A C W W C A T G W
Bpu10I  (789)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NotI  (794)
1 site
G C G G C C G C C G C C G G C G
XbaI  (804)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (900)
1 site
C A A T T G G T T A A C
HpaI  (913)
1 site
G T T A A C C A A T T G
BtsαI  (989)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
AflII  (1032)
1 site
C T T A A G G A A T T C
DraIII  (1266)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SexAI  (1739)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (1925)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (1971)
1 site
A G G C C T T C C G G A
BspDI  (1990)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1990)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
NeoR/KanR
2022 .. 2816  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2022 .. 2816  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
ZsGreen1
97 .. 792  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ZsGreen1
97 .. 792  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ori
3424 .. 4012  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3424 .. 4012  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1042 .. 1497  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1042 .. 1497  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
1630 .. 1987  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
1630 .. 1987  =  358 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
914 .. 1035  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
914 .. 1035  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1524 .. 1628  =  105 bp
AmpR promoter
1524 .. 1628  =  105 bp
MCS
12 .. 89  =  78 bp
multiple cloning site of fluorescent protein plasmids
MCS
12 .. 89  =  78 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3048 .. 3095  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3048 .. 3095  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
SV40 ori
1838 .. 1973  =  136 bp
SV40 origin of replication
SV40 ori
1838 .. 1973  =  136 bp
SV40 origin of replication
ORF:  97 .. 792  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
ORF:  2194 .. 2580  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  2837 .. 3286  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  2022 .. 2816  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  4066 .. 197  =  258 bp
ORF:  85 amino acids
ORF:  474 .. 767  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  2331 .. 2867  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3042 .. 3275  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
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