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pd1EGFP-N1

Vector for fusing destabilized EGFP, with an in vivo half-life of ~1 hour, to the C-terminus of a partner protein.

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pd1EGFP-N1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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AflIII - PciI (4800) ApaLI (4486) EcoO109I (3980) BsaI (3871) RsrII (3398) BsrDI (3115) PflFI - Tth111I (3000) FspI (2984) MscI (2964) PluTI (2885) SfoI (2883) NarI (2882) KasI (2881) BspDI * - ClaI * (2722) StuI (2703) SfiI (2657) SexAI * (2471) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) EcoRI (629) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) BsrGI (1388) BlpI (1399) MreI - SgrAI (1416) NotI (1526) XbaI * (1536) MfeI (1632) HpaI (1645) AflII (1764) DraIII (1998) pd1EGFP-N1 4858 bp
AflIII  (4800)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4800)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4486)
1 site
G T G C A C C A C G T G
EcoO109I  (3980)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different Eco