phMGFP

Vector encoding the Monster Green® fluorescent protein (Monster GFP).

Sequence Author: Promega

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No matches
BglII (4702) PspFI (4390) BseYI (4386) AlwNI (4281) AhdI (3802) BpmI (3733) NmeAIII (3655) XmnI (3202) EcoO109I (2822) NspI (2777) PfoI (2763) SpeI (152) SnaBI (493) Eco53kI (719) SacI (721) HindIII (748) PstI (830) BfuAI - BspMI (844) NheI (1052) BmtI (1056) TspMI - XmaI (1058) SmaI (1060) EcoRV (1066) BclI * (1083) AleI (1250) BstEII (1265) PflFI - Tth111I (1448) BstXI (1627) BsgI (1652) PflMI (1654) NgoMIV (1751) NaeI (1753) XbaI (1764) EagI - NotI (1771) HpaI (1937) MfeI (1946) BsaBI * (2039) BspDI * - ClaI * (2043) phMGFP 4707 bp
BglII  (4702)
1 site
A G A T C T T C T A G A
PspFI  (4390)
1 site
C C C A G C G G G T C G
BseYI  (4386)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (4281)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (3802)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BpmI  (3733)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
NmeAIII  (3655)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XmnI  (3202)
1 site
G A A N N N N T T C C T T N N N N A A G
EcoO109I  (2822)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
NspI  (2777)
1 site
R C A T G Y Y G T A C R
PfoI  (2763)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
SpeI  (152)
1 site
A C T A G T T G A T C A
SnaBI  (493)
1 site
T A C G T A A T G C A T
Eco53kI  (719)
1 site
G A G C T C C T C G A G
SacI  (721)
1 site
G A G C T C C T C G A G
HindIII  (748)
1 site
A A G C T T T T C G A A
PstI  (830)
1 site
C T G C A G G A C G T C
BfuAI  (844)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (844)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
NheI  (1052)
1 site
G C T A G C C G A T C G
BmtI  (1056)
1 site
G C T A G C C G A T C G
TspMI  (1058)
1 site
C C C G G G G G G C C C
XmaI  (1058)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1060)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRV  (1066)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BclI  (1083)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AleI  (1250)
1 site
C A C N N N N G T G G T G N N N N C A C
BstEII  (1265)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PflFI  (1448)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1448)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstXI  (1627)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BsgI  (1652)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PflMI  (1654)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NgoMIV  (1751)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1753)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
XbaI  (1764)
1 site
T C T A G A A G A T C T
EagI  (1771)
1 site
C G G C C G G C C G G C
NotI  (1771)
1 site
G C G G C C G C C G C C G G C G
HpaI  (1937)
1 site
G T T A A C C A A T T G
MfeI  (1946)
1 site
C A A T T G G T T A A C
BsaBI  (2039)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BspDI  (2043)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2043)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
AmpR
3015 .. 3875  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3015 .. 3083  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3015 .. 3875  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3084 .. 3875  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3015 .. 3875  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
hMGFP
1076 .. 1759  =  684 bp
227 amino acids  =  25.9 kDa
Product: Monster Green® fluorescent protein (Monster GFP), a modified version of the GFP from Montastrea cavernosa
mammalian codon-optimized with few consensus transcription factor binding sites
hMGFP
1076 .. 1759  =  684 bp
227 amino acids  =  25.9 kDa
Product: Monster Green® fluorescent protein (Monster GFP), a modified version of the GFP from Montastrea cavernosa
mammalian codon-optimized with few consensus transcription factor binding sites
ori
4046 .. 4634  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4046 .. 4634  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
2123 .. 2578  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2123 .. 2578  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
214 .. 517  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
214 .. 517  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
SV40 poly(A) signal
1816 .. 1937  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1816 .. 1937  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2910 .. 3014  =  105 bp
AmpR promoter
2910 .. 3014  =  105 bp
T7 promoter
1034 .. 1052  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1034 .. 1052  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  1076 .. 1759  =  684 bp
ORF:  227 amino acids  =  25.9 kDa
ORF:  3015 .. 3875  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3479 .. 3745  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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