Resources
Plasmid Files

ratiometric pHluorin

GFP mutant that responds to a drop in pH with a reversible decrease in excitation at 395 nm and a corresponding increase in excitation at 475 nm.

 
To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

 600 400 200 ratiometric pHluorin End (717) PspFI (683) BpuEI (682) AlwNI - BseYI (679) MspA1I - PvuII (676) SmlI (661) DrdI (652) EcoP15I (649) BsaI (638) BstYI (627) BstBI (622) BfuAI - BspMI (608) BsgI (586) BtgZI (581) MfeI (560) KpnI (482) Acc65I - BanI (478) DraIII (447) BsiHKAI (443) DraI (390) AcuI (350) BsaAI - PmlI (325) AflIII (322) NdeI (230) MscI (171) EaeI (169) BtgI - NcoI - StyI (166) BaeGI - Bme1580I (74) BpmI (47) Start (0) ratiometric pHluorin 717 bp
End  (717)
0 sites
PspFI  (683)
1 site
C C C A G C G G G T C G
BpuEI  (682)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (679)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BseYI  (679)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
MspA1I  (676)
1 site
C M G C K G G K C G M C
PvuII  (676)
1 site
C A G C T G G T C G A C
SmlI  (661)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
DrdI  (652)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
EcoP15I  (649)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BsaI  (638)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BstYI  (627)
1 site
R G A T C Y Y C T A G R
BstBI  (622)
1 site
T T C G A A A A G C T T
BfuAI  (608)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (608)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsgI  (586)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BtgZI  (581)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
MfeI  (560)
1 site
C A A T T G G T T A A C
KpnI  (482)
1 site
G G T A C C C C A T G G
Acc65I  (478)
1 site
G G T A C C C C A T G G
BanI  (478)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
DraIII  (447)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsiHKAI  (443)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
DraI  (390)
1 site
T T T A A A A A A T T T
AcuI  (350)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (325)
1 site
Y A C G T R R T G C A Y
PmlI  (325)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AflIII  (322)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
NdeI  (230)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
MscI  (171)
1 site
T G G C C A A C C G G T
EaeI  (169)
1 site
Y G G C C R R C C G G Y
BtgI  (166)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (166)
1 site
C C A T G G G G T A C C
StyI  (166)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BaeGI  (74)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (74)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BpmI  (47)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Start  (0)
0 sites
ratiometric pHluorin
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: pH-sensitive mutant of green fluorescent
protein (Miesenböck et al., 1998)
responds to a drop in pH with a reversible decrease
in excitation at 395 nm and a corresponding
increase in excitation at 475 nm
ratiometric pHluorin
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: pH-sensitive mutant of green fluorescent
protein (Miesenböck et al., 1998)
responds to a drop in pH with a reversible decrease
in excitation at 395 nm and a corresponding
increase in excitation at 475 nm
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter