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Plasmid Files

pAd/CMV/V5-DEST™

Adenoviral Gateway® destination vector for constitutive expression of a C-terminally V5-tagged protein.

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pAd CMV V5-DEST Sequence and MappAd CMV V5-DEST.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
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 5' ITR PacI (36,683) PacI (34,609) MreI (26,932) AsiSI (21,985) Ad5 Ψ CMV promoter T7 promoter attR1 V5 tag HSV TK poly(A) signal pAd/CMV/V5-DEST™ 36,686 bp
PacI  (36,683)
2 sites
T T A A T T A A A A T T A A T T
PacI  (34,609)
2 sites
T T A A T T A A A A T T A A T T
MreI  (26,932)
1 site
C G C C G G C G G C G G C C G C
AsiSI  (21,985)
1 site
G C G A T C G C C G C T A G C G
adenoviral DNA
4056 .. 34,604  =  30,549 bp
sequences from human adenovirus serotype 5,
lacking the E1 and E3 regions
adenoviral DNA
4056 .. 34,604  =  30,549 bp
sequences from human adenovirus serotype 5,
lacking the E1 and E3 regions
AmpR
35,568 .. 36,428  =  861 bp
286 amino acids  =  31.4 kDa
   Segment 2:  
   35,568 .. 36,359  =  792 bp
   263 amino acids  =  28.8 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
35,568 .. 36,428  =  861 bp
286 amino acids  =  31.4 kDa
   Segment 1:  signal sequence  
   36,360 .. 36,428  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
35,568 .. 36,428  =  861 bp
286 amino acids  =  31.4 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
CmR
2607 .. 3266  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
2607 .. 3266  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
34,828 .. 35,416  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
34,828 .. 35,416  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CMV enhancer
731 .. 1110  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
731 .. 1110  =  380 bp
human cytomegalovirus immediate early enhancer
ccdB
1960 .. 2265  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
ccdB
1960 .. 2265  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
CMV promoter
1111 .. 1314  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
1111 .. 1314  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
attR1
1407 .. 1531  =  125 bp
recombination site for the Gateway® LR reaction
attR1
1407 .. 1531  =  125 bp
recombination site for the Gateway® LR reaction
attR2
3547 .. 3671  =  125 bp
recombination site for the Gateway® LR reaction
attR2
3547 .. 3671  =  125 bp
recombination site for the Gateway® LR reaction
AmpR promoter
36,429 .. 36,533  =  105 bp
AmpR promoter
36,429 .. 36,533  =  105 bp
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
cat promoter
3267 .. 3369  =  103 bp
promoter of the E. coli cat gene
cat promoter
3267 .. 3369  =  103 bp
promoter of the E. coli cat gene
HSV TK poly(A) signal
3822 .. 3870  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3822 .. 3870  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
V5 tag
3697 .. 3738  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
3697 .. 3738  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
T7 promoter
1359 .. 1377  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1359 .. 1377  =  19 bp
promoter for bacteriophage T7 RNA polymerase
3' ITR
34,502 .. 34,604  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
3' ITR
34,502 .. 34,604  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
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