Resources
Plasmid Files

pDEST™26

Gateway® destination vector for high-level expression of N-terminally 6xHis-tagged proteins in mammalian cells.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pDEST26 Sequence and MappDEST26.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 CMV enhancer AflIII - PciI (7092) AhdI (6204) XmnI (5604) PfoI (5165) BstBI (4910) RsrII (4744) PflFI - Tth111I (4346) PluTI (4231) SfoI (4229) NarI (4228) KasI (4227) AflII (4082) HindIII (4050) StuI (4033) BseRI (4030) SfiI (3987) SexAI * (3801) KpnI (3688) SnaBI (294) Eco53kI (520) SacI (522) BbsI (588) SacII (626) ATG 6xHis XbaI * (662) NotI (797) EcoRI (1117) PasI - PflMI * (1349) BglII (1564) BstZ17I (1652) BbvCI (1842) AvaI - BsoBI - TspMI - XmaI (1986) BmeT110I (1987) SmaI - SrfI (1988) BmgBI (2022) SalI (2245) M13 fwd NheI (2475) BmtI (2479) BclI * (2751) MfeI (2844) HpaI (2857) DraIII (3351) Acc65I (3684) pDEST™26 7481 bp
AflIII  (7092)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (7092)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (6204)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
XmnI  (5604)
1 site
G A A N N N N T T C C T T N N N N A A G
PfoI  (5165)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (4910)
1 site
T T C G A A A A G C T T
RsrII  (4744)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (4346)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4346)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (4231)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (4229)
1 site
G G C G C C C C G C G G
NarI  (4228)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (4227)
1 site
G G C G C C C C G C G G
AflII  (4082)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
HindIII  (4050)
1 site
A A G C T T T T C G A A
StuI  (4033)
1 site
A G G C C T T C C G G A
BseRI  (4030)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
SfiI  (3987)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SexAI  (3801)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
KpnI  (3688)
1 site
G G T A C C C C A T G G
SnaBI  (294)
1 site
T A C G T A A T G C A T
Eco53kI  (520)
1 site
G A G C T C C T C G A G
SacI  (522)
1 site
G A G C T C C T C G A G
BbsI  (588)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
SacII  (626)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
XbaI  (662)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
NotI  (797)
1 site
G C G G C C G C C G C C G G C G
EcoRI  (1117)
1 site
G A A T T C C T T A A G
PasI  (1349)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (1349)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BglII  (1564)
1 site
A G A T C T T C T A G A
BstZ17I  (1652)
1 site
G T A T A C C A T A T G
BbvCI  (1842)
1 site
C C T C A G C G G A G T C G
AvaI  (1986)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1986)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
TspMI  (1986)
1 site
C C C G G G G G G C C C
XmaI  (1986)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BmeT110I  (1987)
1 site
C Y C G R G G R G C Y C
SmaI  (1988)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SrfI  (1988)
1 site
G C C C G G G C C G G G C C C G
BmgBI  (2022)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
SalI  (2245)
1 site
G T C G A C C A G C T G
NheI  (2475)
1 site
G C T A G C C G A T C G
BmtI  (2479)
1 site
G C T A G C C G A T C G
BclI  (2751)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (2844)
1 site
C A A T T G G T T A A C
HpaI  (2857)
1 site
G T T A A C C A A T T G
DraIII  (3351)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
Acc65I  (3684)
1 site
G G T A C C C C A T G G
AmpR
5417 .. 6277  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   5417 .. 5485  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
5417 .. 6277  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   5486 .. 6277  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
5417 .. 6277  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
NeoR/KanR
4100 .. 4894  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
4100 .. 4894  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
CmR
904 .. 1563  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
904 .. 1563  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
6448 .. 7036  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
6448 .. 7036  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
3118 .. 3573  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
3118 .. 3573  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
3692 .. 4049  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
3692 .. 4049  =  358 bp
SV40 enhancer and early promoter
ccdB
1905 .. 2210  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
ccdB
1905 .. 2210  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
CMV enhancer
15 .. 318  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
15 .. 318  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
319 .. 522  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
319 .. 522  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
2858 .. 2992  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2858 .. 2992  =  135 bp
SV40 polyadenylation signal
attR1
671 .. 795  =  125 bp
recombination site for the Gateway® LR reaction
attR1
671 .. 795  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2251 .. 2375  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2251 .. 2375  =  125 bp
recombination site for the Gateway® LR reaction
AmpR promoter
5312 .. 5416  =  105 bp
AmpR promoter
5312 .. 5416  =  105 bp
poly(A) signal
4958 .. 5006  =  49 bp
synthetic polyadenylation signal
poly(A) signal
4958 .. 5006  =  49 bp
synthetic polyadenylation signal
lac UV5 promoter
820 .. 850  =  31 bp
   Segment 1:  -35  
   820 .. 825  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
820 .. 850  =  31 bp
   Segment 2:  
   826 .. 843  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
820 .. 850  =  31 bp
   Segment 3:  -10  
   844 .. 850  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
820 .. 850  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
T7 promoter
2408 .. 2426  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2408 .. 2426  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
644 .. 661  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
644 .. 661  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
M13 fwd
2437 .. 2453  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
2437 .. 2453  =  17 bp
common sequencing primer, one of multiple similar
variants
ATG
632 .. 634  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
632 .. 634  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 ori
3900 .. 4035  =  136 bp
SV40 origin of replication
SV40 ori
3900 .. 4035  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter