Resources
Plasmid Files

pIB/V5-His-DEST

Gateway® destination vector for stable constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pIB V5-His-DEST.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
Download Free Trial Get SnapGene Viewer

´╗┐
AhdI (4870) BglI (4752) XmnI (4270) EarI (4071) BglII (4003) DraIII (3970) BlpI (3960) NruI * (3796) PpuMI (3724) MreI - SgrAI (3658) BbsI (3650) SpeI (3415) BciVI (2826) PciI (2623) BspDI * - ClaI * (2560) BspHI (0) FseI (293) BsiWI (317) RsrII (335) KasI (474) NarI (475) SfoI (476) PluTI (478) HindIII (557) EcoRV (565) AfeI (586) NotI (728) BspEI (1044) BsmBI (1272) PasI - PflMI * (1280) SspI (1360) BssHII (1542) BbvCI (1773) SrfI (1919) BstXI (2036) BfuAI - BspMI (2163) SalI (2176) PshAI (2315) XbaI (2319) SacII (2332) AgeI (2386) PsiI (2493) pIB/V5-His-DEST 5038 bp
AhdI  (4870)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BglI  (4752)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
XmnI  (4270)
1 site
G A A N N N N T T C C T T N N N N A A G
EarI  (4071)
1 site
C T C T T C N G A G