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pYES-DEST52

Gateway® destination vector for galactose-inducible high-level expression of proteins in Saccharomyces cerevisiae.

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pYES-DEST52.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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NaeI (7476) NgoMIV (7474) SwaI (7148) SnaBI (6844) HpaI (6199) PfoI * (6097) NruI (5793) BlpI (5757) BspDI - ClaI (5613) BmtI (5608) NheI (5604) NdeI (5464) BsgI (5435) SbfI (5401) XcmI (5252) EcoRV (5191) BsaHI (4231) BglI (3814) SpeI (7616) SacII (643) BspEI (980) EcoRI (984) PasI - PflMI * (1216) BssHII (1478) BbvCI (1709) TspMI - XmaI (1853) SmaI - SrfI (1855) BsaBI * (1868) BstXI (1972) SalI (2112) PmeI (2340) BciVI (3004) BspHI (3521) AhdI (3694) pYES-DEST52 7621 bp
NaeI  (7476)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (7474)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SwaI  (7148)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
SnaBI  (6844)
1 site
T A C G T A A T G C A T
HpaI  (6199)
1 site
G T T A A C C A A T T G
PfoI  (6097)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
NruI  (5793)
1 site
T C G C G A A G C G C T
BlpI  (5757)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BspDI  (5613)
1 site
A T C G A T T A G C T A
ClaI  (5613)
1 site
A T C G A T T A G C