Resources
Plasmid Files

pcDNA™6.2/V5-DEST

Gateway® destination vector for high-level expression of C-terminally V5-tagged proteins in mammalian cells.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pcDNA6.2 V5-DEST Sequence and MappcDNA6.2 V5-DEST.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 SgrDI (7339) PciI (5528) BspQI - SapI (5412) PfoI (4952) BlpI (4856) BsgI (4648) PpuMI (4620) EagI (4577) BbsI (4546) BclI * (4383) AvrII (4332) StuI (4331) SexAI * (4099) MfeI (161) SpeI (249) CMV enhancer NdeI (484) SnaBI (590) Eco53kI (816) SacI (818) PstI (1434) BfuAI - BspMI (1437) BstXI (1572) BmgBI (1651) SrfI (1685) BbvCI (1828) BssHII (2058) BamHI (2099) EcoRI (2552) BspEI (2556) XbaI * (3179) PspOMI (3185) ApaI (3189) SacII (3192) BstBI (3196) AgeI (3246) PmeI (3264) pcDNA™6.2/V5-DEST 7341 bp
SgrDI  (7339)
1 site
C G T C G A C G G C A G C T G C
PciI  (5528)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5412)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5412)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
PfoI  (4952)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BlpI  (4856)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsgI  (4648)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PpuMI  (4620)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EagI  (4577)
1 site
C G G C C G G C C G G C
BbsI  (4546)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BclI  (4383)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AvrII  (4332)
1 site
C C T A G G G G A T C C
StuI  (4331)
1 site
A G G C C T T C C G G A
SexAI  (4099)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MfeI  (161)
1 site
C A A T T G G T T A A C
SpeI  (249)
1 site
A C T A G T T G A T C A
NdeI  (484)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (590)
1 site
T A C G T A A T G C A T
Eco53kI  (816)
1 site
G A G C T C C T C G A G
SacI  (818)
1 site
G A G C T C C T C G A G
PstI  (1434)
1 site
C T G C A G G A C G T C
BfuAI  (1437)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1437)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BstXI  (1572)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BmgBI  (1651)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
SrfI  (1685)
1 site
G C C C G G G C C G G G C C C G
BbvCI  (1828)
1 site
C C T C A G C G G A G T C G
BssHII  (2058)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BamHI  (2099)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (2552)
1 site
G A A T T C C T T A A G
BspEI  (2556)
1 site
T C C G G A A G G C C T
XbaI  (3179)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
PspOMI  (3185)
1 site
G G G C C C C C C G G G
ApaI  (3189)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SacII  (3192)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BstBI  (3196)
1 site
T T C G A A A A G C T T
AgeI  (3246)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
PmeI  (3264)
1 site
G T T T A A A C C A A A T T T G
AmpR
6345 .. 7205  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   6345 .. 7136  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6345 .. 7205  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   7137 .. 7205  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6345 .. 7205  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
CmR
2111 .. 2770  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
2111 .. 2770  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
5589 .. 6174  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
5589 .. 6174  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
3576 .. 4004  =  429 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
3576 .. 4004  =  429 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
BSD
4461 .. 4859  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
4461 .. 4859  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
4018 .. 4347  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
4018 .. 4347  =  330 bp
SV40 enhancer and early promoter
ccdB
1464 .. 1769  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
ccdB
1464 .. 1769  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
attR1
911 .. 1035  =  125 bp
recombination site for the Gateway® LR reaction
attR1
911 .. 1035  =  125 bp
recombination site for the Gateway® LR reaction
attR2
3051 .. 3175  =  125 bp
recombination site for the Gateway® LR reaction
attR2
3051 .. 3175  =  125 bp
recombination site for the Gateway® LR reaction
SV40 poly(A) signal
5017 .. 5138  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5017 .. 5138  =  122 bp
SV40 polyadenylation signal
AmpR promoter
7206 .. 7310  =  105 bp
AmpR promoter
7206 .. 7310  =  105 bp
cat promoter
2771 .. 2873  =  103 bp
promoter of the E. coli cat gene
cat promoter
2771 .. 2873  =  103 bp
promoter of the E. coli cat gene
HSV TK poly(A) signal
3326 .. 3374  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3326 .. 3374  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
EM7 promoter
4395 .. 4442  =  48 bp
synthetic bacterial promoter
EM7 promoter
4395 .. 4442  =  48 bp
synthetic bacterial promoter
V5 tag
3201 .. 3242  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
3201 .. 3242  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
4198 .. 4333  =  136 bp
SV40 origin of replication
SV40 ori
4198 .. 4333  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter