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Plasmid Files

pAMP1

Bacterial vector for inducible expression of a cloned cDNA. For an MCS with FseI and SalI sites, use pAMP10.

To see this sequence with restriction sites, features, and translations, please download
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pAMP1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  I.M.A.G.E. Consortium
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NaeI (3985) NgoMIV (3983) DraIII (3882) PsiI (3754) XmnI (3338) ScaI (3219) TatI (3217) BsaI (2800) AhdI (2739) AlwNI (2262) ZraI (193) AatII (195) SphI (201) MluI (203) BsiWI (206) SnaBI (210) HindIII (213) BamHI (219) XbaI (225) EagI - NotI (232) AvaI - BsoBI - TspMI - XmaI (263) BmeT110I (264) SmaI (265) EcoRI (268) BspEI (272) RsrII (275) AgeI (277) Acc65I (280) KpnI (284) PstI - SbfI (289) BfuAI - BspMI (292) KasI (557) NarI * (558) SfoI (559) PluTI (561) BsmBI (581) EcoRV (750) PspOMI (989) ApaI (993) BstEII (1014) BclI * (1182) PflMI (1621) MauBI (1653) PciI (1846) pAMP1 4114 bp
NaeI  (3985)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3983)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (3882)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (3754)
1 site
T T A T A A A A T A T T
XmnI  (3338)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3219)
1 site
A G T A C T T C A T G A
TatI  (3217)
1 site
W G T A C W W C A T G W
BsaI  (2800)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2739)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2262)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ZraI  (193)
1 site
G A C G T C C T G C A G
AatII  (195)
1 site
G A C G T C C T G C A G
SphI  (201)
1 site
G C A T G C C G T A C G
MluI  (203)
1 site
A C G C G T T G C G C A
BsiWI  (206)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (210)
1 site
T A C G T A A T G C A T
HindIII  (213)
1 site
A A G C T T T T C G A A
BamHI  (219)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (225)
1 site
T C T A G A A G A T C T
EagI  (232)
1 site
C G G C C G G C C G G C
NotI  (232)
1 site
G C G G C C G C C G C C G G C G
AvaI  (263)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (263)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (263)
1 site
C C C G G G G G G C C C
XmaI  (263)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BmeT110I  (264)
1 site
C Y C G R G G R G C Y C
SmaI  (265)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (268)
1 site
G A A T T C C T T A A G
BspEI  (272)
1 site
T C C G G A A G G C C T
RsrII  (275)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AgeI  (277)
1 site
A C C G G T T G G C C A
Acc65I  (280)
1 site
G G T A C C C C A T G G
KpnI  (284)
1 site
G G T A C C C C A T G G
PstI  (289)
1 site
C T G C A G G A C G T C
SbfI  (289)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (292)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (292)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
KasI  (557)
1 site
G G C G C C C C G C G G
NarI  (558)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (559)
1 site
G G C G C C C C