Resources
Plasmid Files

pBluescribe (modified)

Bacterial vector for cloning cDNAs as MluI-SalI fragments.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pBluescribe (modified) Sequence and MappBluescribe (modified).dna
Map and Sequence File   
Sequence Author:  I.M.A.G.E. Consortium
Download Free Trial Get SnapGene Viewer

 MluI (2731) PluTI (2529) SfoI (2527) NarI (2526) KasI (2525) BstAPI (2475) NdeI (2474) PfoI (2336) EcoO109I (2279) AatII (2225) ZraI (2223) SspI (2107) XmnI (1902) ScaI (1783) TsoI (1702) NmeAIII (1451) BsrFI (1383) BpmI (1373) SalI (10) AccI (11) HincII (12) M13 rev lac operator BspQI - SapI (294) PciI (410) BseYI (714) PspFI (718) AlwNI (826) AhdI (1303) BsaI (1364) pBluescribe (modified) 2738 bp
MluI  (2731)
1 site
A C G C G T T G C G C A
PluTI  (2529)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (2527)
1 site
G G C G C C C C G C G G
NarI  (2526)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (2525)
1 site
G G C G C C C C G C G G
BstAPI  (2475)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
NdeI  (2474)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
PfoI  (2336)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
EcoO109I  (2279)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (2225)
1 site
G A C G T C C T G C A G
ZraI  (2223)
1 site
G A C G T C C T G C A G
SspI  (2107)
1 site
A A T A T T T T A T A A
XmnI  (1902)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (1783)
1 site
A G T A C T T C A T G A
TsoI  (1702)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (1451)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (1383)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI  (1373)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
SalI  (10)
1 site
G T C G A C C A G C T G
AccI  (11)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (12)
1 site
G T Y R A C C A R Y T G
BspQI  (294)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (294)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
PciI  (410)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BseYI  (714)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (718)
1 site
C C C A G C G G G T C G
AlwNI  (826)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (1303)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (1364)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AmpR
1230 .. 2090  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1230 .. 2021  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1230 .. 2090  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   2022 .. 2090  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1230 .. 2090  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
471 .. 1059  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
471 .. 1059  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
AmpR promoter
2091 .. 2195  =  105 bp
AmpR promoter
2091 .. 2195  =  105 bp
lac promoter
117 .. 147  =  31 bp
   Segment 3:  -10  
   117 .. 123  =  7 bp
promoter for the E. coli lac operon
lac promoter
117 .. 147  =  31 bp
   Segment 2:  
   124 .. 141  =  18 bp
promoter for the E. coli lac operon
lac promoter
117 .. 147  =  31 bp
   Segment 1:  -35  
   142 .. 147  =  6 bp
promoter for the E. coli lac operon
lac promoter
117 .. 147  =  31 bp
3 segments
promoter for the E. coli lac operon
T3 promoter
30 .. 48  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T3 promoter
30 .. 48  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T7 promoter
2692 .. 2710  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2692 .. 2710  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 rev
69 .. 85  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
69 .. 85  =  17 bp
common sequencing primer, one of multiple similar
variants
lac operator
93 .. 109  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
93 .. 109  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
M13 fwd
2669 .. 2685  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
2669 .. 2685  =  17 bp
common sequencing primer, one of multiple similar
variants
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter