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pCMV SPORT2

Vector for cloning and transient mammalian cell expression of cDNAs.

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pCMV SPORT2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  I.M.A.G.E. Consortium
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NaeI (4345) NgoMIV (4343) DraIII (4242) PflMI * (3930) XmnI (3542) ScaI (3423) BsaI (3004) AhdI (2943) AlwNI (2424) PspFI (2316) BseYI (2312) BspDI * - ClaI * (123) HpaI (259) MfeI (268) BsaBI * (360) BclI * (361) NheI (637) BmtI (641) BglII (643) SphI (731) MluI (733) HindIII (739) PspOMI (745) ApaI (749) AbsI - PaeR7I - PspXI - XhoI (751) BamHI (757) XbaI (763) EagI - NotI (770) SpeI (779) SalI (791) TspMI - XmaI (796) SmaI (798) EcoRI (801) RsrII (808) AgeI (810) Acc65I (813) KpnI (817) PstI - SbfI (822) SacII (912) AvrII (915) BbsI (944) BsmBI (989) NcoI (1218) SnaBI (1242) BsgI * (1510) BstZ17I (1641) BstAPI (1832) BspQI - SapI (1892) PciI (2008) pCMV SPORT2 4474 bp
NaeI  (4345)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4343)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (4242)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PflMI  (3930)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
XmnI  (3542)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3423)
1 site
A G T A C T T C A T G A
BsaI  (3004)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2943)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2424)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (2316)
1 site
C C C A G C G G G T C G
BseYI  (2312)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspDI  (123)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (123)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
HpaI  (259)
1 site
G T T A A C C A A T T G
MfeI  (268)
1 site
C A A T T G G T T A A C
BsaBI  (360)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BclI  (361)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
NheI  (637)
1 site
G C T A G C C G A T C G
BmtI  (641)
1 site
G C T A G C C G A T C G
BglII  (643)
1 site
A G A T C T T C T A G A
SphI  (731)
1 site
G C A T G C C G T A C G
MluI  (733)
1 site
A C G C G T T G C G C A
HindIII  (739)
1 site
A A G C T T T T C G A A
PspOMI  (745)
1 site
G G G C C C C C C G G G
ApaI  (749)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AbsI  (751)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (751)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (751)
1 site
V C T C G A G B B G A G C T C V
XhoI  (751)
1 site
C T C G A G G A G C T C
BamHI  (757)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (763)
1 site
T C T A G A A G A T C T
EagI  (770)
1 site
C G G C C G G C C G G C
NotI  (770)
1 site
G C G G C C G C C G C C G G C G
SpeI  (779)
1 site
A C T A G T T G A T C A
SalI  (791)
1 site
G T C G A C C A G C T G
TspMI  (796)
1 site
C C C G G G G G G C C C
XmaI  (796)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (798)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (801)
1 site
G A A T T C C T T A A G
RsrII  (808)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AgeI  (810)
1 site
A C C G G T T G G C C A
Acc65I  (813)
1 site
G G T A C C C C A T G G
KpnI  (817)
1 site
G G T A C C C C A T G G
PstI  (822)
1 site
C T G C A G G A C G T C
SbfI  (822)
1 site
C C T G C A G G G G A C G T C C
SacII  (912)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AvrII  (915)
1 site
C C T A G G G G A T C C
BbsI  (944)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsmBI  (989)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
NcoI  (1218)
1 site
C C A T G G G G T A C C
SnaBI  (1242)
1 site
T A C G T A A T G C A T
BsgI  (1510)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
* Blocked by EcoKI methylation.
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).