Resources
Plasmid Files

pDNR-LIB

Donor vector for transferring a cloned cDNA into an acceptor vector using Cre recombinase in the Creator™ system.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pDNR-LIB.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
Download Free Trial Get SnapGene Viewer


BmtI (4114) NheI (4110) BglII (4082) MluI (4059) BssS α I (3831) BciVI (3806) PspFI (3704) BseYI (3700) ApaLI (3690) AlwNI (3595) BspHI (3284) AscI (3170) BfuAI - BspMI (3148) BstZ17I (2851) EarI (2486) StuI * (2481) MslI (2240) BsgI (2159) SalI (45) NdeI (61) TspMI - XmaI (66) SmaI (68) EcoRI (71) PstI - SbfI (93) MfeI (148) NmeAIII (285) PaeR7I - XhoI (311) HindIII (317) XbaI (323) BstXI (336) BmrI (344) PspOMI (346) ApaI (350) AvrII (384) SpeI (403) Eco53kI (517) SacI (519) ScaI (558) SspI (663) NcoI (670) PasI (740) PflMI * (746) BsmBI (747) BpmI (855) BsrDI (958) BspEI (975) ZraI (1262) AatII (1264) SacII (1645) BsaAI - SnaBI (1973) BsrGI (2038) pDNR-LIB 4161 bp
BmtI  (4114)
1 site
G C T A G C C G A T C G
NheI  (4110)
1 site
G C T A G C C G A T C G
BglII  (4082)
1 site
A G A T C T T C T A G A
MluI  (4059)
1 site
A C G C G T T G C G C A
BssSαI  (3831)
1 site
C A C G A G G T G C T C
BciVI  (3806)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
PspFI  (3704)
1 site
C C C A G C G G G T C G
BseYI  (3700)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
ApaLI  (3690)
1 site
G T G C A C C A C G T G
AlwNI  (3595)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BspHI  (3284)
1 site
T C A T G A A G T A C T
AscI  (3170)
1 site
G G C G C G C C C C G C G C G G