Resources
Plasmid Files

pOTB7a

cDNA cloning vector derived from pOTB7.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pOTB7a Sequence and MappOTB7a.dna
Map and Sequence File   
Sequence Author:  I.M.A.G.E. Consortium
Download Free Trial Get SnapGene Viewer

 SalI - SgrDI (1800) ScaI (1763) SspI (1658) BtgI - NcoI - StyI (1647) MscI (1613) PasI - PflMI * (1578) BsmBI (1570) BpmI (1468) AclI (1433) BsrDI (1365) BspEI (1342) TsoI (1247) PvuII (1246) Bpu10I (1119) EagI - NotI (1080) BspDI - ClaI (1068) DrdI (946) AccI (1801) BamHI (79) M13 fwd Bsu36I (141) I-CeuI (144) StuI * (154) BglI - SfiI (160) PaeR7I - PspXI - XhoI (165) EcoRI (177) XmnI (181) BanI (240) BglII (267) BsrFI (273) BsaI (282) HpaI (318) BfuAI - BspMI (329) PstI (343) TspMI - XmaI (347) SmaI (349) AcuI (506) AlwNI (639) ApaLI (734) BaeGI - Bme1580I - BsiHKAI - Bsp1286I (738) HaeII (808) BciVI (850) BssS α I (875) pOTB7a 1804 bp
SalI  (1800)
1 site
G T C G A C C A G C T G
SgrDI  (1800)
1 site
C G T C G A C G G C A G C T G C
ScaI  (1763)
1 site
A G T A C T T C A T G A
SspI  (1658)
1 site
A A T A T T T T A T A A
BtgI  (1647)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (1647)
1 site
C C A T G G G G T A C C
StyI  (1647)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (1613)
1 site
T G G C C A A C C G G T
PasI  (1578)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (1578)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BsmBI  (1570)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BpmI  (1468)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AclI  (1433)
1 site
A A C G T T T T G C A A
BsrDI  (1365)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BspEI  (1342)
1 site
T C C G G A A G G C C T
TsoI  (1247)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuII  (1246)
1 site
C A G C T G G T C G A C
Bpu10I  (1119)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EagI  (1080)
1 site
C G G C C G G C C G G C
NotI  (1080)
1 site
G C G G C C G C C G C C G G C G
BspDI  (1068)
1 site
A T C G A T T A G C T A
ClaI  (1068)
1 site
A T C G A T T A G C T A
DrdI  (946)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AccI  (1801)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BamHI  (79)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
Bsu36I  (141)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
I-CeuI  (144)
1 site
T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T

I-CeuI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
StuI  (154)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
BglI  (160)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (160)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PaeR7I  (165)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (165)
1 site
V C T C G A G B B G A G C T C V
XhoI  (165)
1 site
C T C G A G G A G C T C
EcoRI  (177)
1 site
G A A T T C C T T A A G
XmnI  (181)
1 site
G A A N N N N T T C C T T N N N N A A G
BanI  (240)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BglII  (267)
1 site
A G A T C T T C T A G A
BsrFI  (273)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BsaI  (282)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
HpaI  (318)
1 site
G T T A A C C A A T T G
BfuAI  (329)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (329)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PstI  (343)
1 site
C T G C A G G A C G T C
TspMI  (347)
1 site
C C C G G G G G G C C C
XmaI  (347)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (349)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
AcuI  (506)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (639)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (734)
1 site
G T G C A C C A C G T G
BaeGI  (738)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (738)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsiHKAI  (738)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (738)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
HaeII  (808)
1 site
R G C G C Y Y C G C G R
BciVI  (850)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSαI  (875)
1 site
C A C G A G G T G C T C
CmR
1133 .. 1792  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1133 .. 1792  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
404 .. 992  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
404 .. 992  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
attB1
85 .. 109  =  25 bp
recombination site for the Gateway® BP reaction
attB1
85 .. 109  =  25 bp
recombination site for the Gateway® BP reaction
SP6 promoter
53 .. 71  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
53 .. 71  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
T7 promoter
282 .. 300  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
282 .. 300  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 fwd
111 .. 127  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
111 .. 127  =  17 bp
common sequencing primer, one of multiple similar
variants
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter