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Plasmid Files

pAB-GST™

Baculovirus transfer plasmid for high-level expression of proteins with an HRV 3C-cleavable N-terminal GST tag.

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pAB-GST.dna
Map and Sequence File:    Download    Open   
Sequence Author:  AB Vector
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FauNDI - NdeI (10,121) BpmI - GsuI (9020) AlwNI - CaiI (8473) GsaI - PspFI (8365) BseYI (8361) FspAI (7024) SgrDI (6871) SgrAI (6602) BstSNI - Eco105I - SnaBI (5514) KpnI (5334) Acc65I - Asp718I (5330) PacI (583) XcmI (746) BstDSI - BtgI (868) Cfr42I - KspI - SacII - Sfr303I - SgrBI - SstII (871) BstEII - BstPI - Eco91I - EcoO65I - PspEI (923) BssT1I - Eco130I - EcoT14I - ErhI - StyI (1501) BbuI - PaeI - SphI (2135) BbvCI - Bpu10I (3405) MroNI - NgoMIV (3770) NaeI - PdiI (3772) Eco32I - EcoRV (4000) BstENI - EcoNI - XagI (4148) BamHI (4825) EcoRI (4834) Cfr9I - TspMI - XmaI - XmaCI (4839) SmaI (4841) BseX3I - BstZI - CciNI - EagI - EclXI - Eco52I - NotI (4855) BglII - PstI (4865) KflI - PpuMI - Psp5II - PspPPI - SanDI (4883) pAB-GST™ 10,335 bp
FauNDI  (10,121)
1 site
C A T A T G G T A T A C
NdeI  (10,121)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BpmI  (9020)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
GsuI  (9020)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Sticky ends from different GsuI sites may not be compatible.
After cleavage, GsuI can remain bound to DNA and alter its electrophoretic mobility.
GsuI is typically used at 30°C, but is 70% active at 37°C.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (8473)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
CaiI  (8473)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different CaiI sites may not be compatible.
GsaI  (8365)
1 site
C C C A G C G G G T C G
PspFI  (8365)
1 site
C C C A G C G G G T C G
BseYI  (8361)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
FspAI  (7024)
1 site
R T G C G C A Y Y A C G C G T R
SgrDI  (6871)
1 site
C G T C G A C G G C A G C T G C
SgrAI  (6602)
1 site
C R C C G G Y G G Y G G C C