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Plasmid Files

pAB-bee™-8xHis

Baculovirus transfer plasmid for high-level expression of secreted proteins with N- and C-terminal 8xHis tags.

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pAB-bee-8xHis.dna
Map and Sequence File:    Download    Open   
Sequence Author:  AB Vector
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NdeI (9751) ScaI (9060) AlwNI (8103) PspFI (7995) BseYI (7991) BspQI - SapI (7571) FspAI (6654) SgrDI (6501) SgrAI (6232) SnaBI (5144) PacI (583) XcmI (746) BtgI (868) SacII (871) BstEII (923) PspOMI (1395) ApaI - BanII (1399) PaeR7I - PspXI - XhoI (1901) SphI (2135) BclI * (2232) BbvCI - Bpu10I (3405) NgoMIV (3770) NaeI (3772) EcoRV (4000) BglII (4134) BstZ17I (4184) EagI - NotI (4237) XbaI (4252) TspMI - XmaI (4263) SmaI (4265) BamHI (4267) AvrII (4488) KflI - PpuMI (4513) pAB-bee™-8xHis 9965 bp
NdeI  (9751)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
ScaI  (9060)
1 site
A G T A C T T C A T G A
AlwNI  (8103)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (7995)
1 site
C C C A G C G G G T C G
BseYI  (7991)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (7571)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7571)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (6654)
1 site
R T G C G C A Y Y A C G C G T R
SgrDI  (6501)
1 site
C G T C G A C G G C A G C T G C
SgrAI  (6232)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SnaBI  (5144)
1 site
T A C G T A A T G C A T
PacI  (583)
1 site
T T A A T T A A A A T T A A T T
XcmI  (746)
1 site
C C A N N N N N N