pBACgus-1

Baculovirus transfer plasmid with the polyhedrin promoter, encoding β-glucuronidase plus an optional C-terminal 6xHis tag.

Sequence Author: MilliporeSigma (Novagen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
DraIII (7116) ScaI (6444) NmeAIII (6112) BpmI (6034) PspFI (5379) BseYI (5375) PmeI (4951) SgrAI (4663) AgeI (4423) BmgBI (4079) AatII (3856) ZraI (3854) BstXI (315) NsiI (332) BbvCI - Bpu10I (564) baculovirus recombination region (lef2/ORF603) BstBI (1882) MscI (2356) PshAI (2404) BtgI (2411) BglII (3310) BamHI (3412) StuI * (3420) EcoRI (3424) Eco53kI (3432) SacI (3434) HindIII (3437) EagI - NotI (3444) AvaI - BsoBI - PaeR7I - PspXI - XhoI (3452) 6xHis AvrII - StyI (3479) BlpI (3488) SphI (3498) SwaI (3665) pBACgus-1 7408 bp
DraIII  (7116)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (6444)
1 site
A G T A C T T C A T G A
NmeAIII  (6112)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (6034)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PspFI  (5379)
1 site
C C C A G C G G G T C G
BseYI  (5375)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PmeI  (4951)
1 site
G T T T A A A C C A A A T T T G
SgrAI  (4663)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AgeI  (4423)
1 site
A C C G G T T G G C C A
BmgBI  (4079)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
AatII  (3856)
1 site
G A C G T C C T G C A G
ZraI  (3854)
1 site
G A C G T C C T G C A G
BstXI  (315)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NsiI  (332)
1 site
A T G C A T T A C G T A
BbvCI  (564)
1 site
C C T C A G C G G A G T C G
Bpu10I  (564)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstBI  (1882)
1 site
T T C G A A A A G C T T
MscI  (2356)
1 site
T G G C C A A C C G G T
PshAI  (2404)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BtgI  (2411)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BglII  (3310)
1 site
A G A T C T T C T A G A
BamHI  (3412)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
StuI  (3420)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
EcoRI  (3424)
1 site
G A A T T C C T T A A G
Eco53kI  (3432)
1 site
G A G C T C C T C G A G
SacI  (3434)
1 site
G A G C T C C T C G A G
HindIII  (3437)
1 site
A A G C T T T T C G A A
EagI  (3444)
1 site
C G G C C G G C C G G C
NotI  (3444)
1 site
G C G G C C G C C G C C G G C G
AvaI  (3452)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (3452)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (3452)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3452)
1 site
V C T C G A G B B G A G C T C V
XhoI  (3452)
1 site
C T C G A G G A G C T C
AvrII  (3479)
1 site
C C T A G G G G A T C C
StyI  (3479)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BlpI  (3488)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SphI  (3498)
1 site
G C A T G C C G T A C G
SwaI  (3665)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
GUS
1167 .. 2978  =  1812 bp
603 amino acids  =  68.4 kDa
Product: β-glucuronidase
GUS
1167 .. 2978  =  1812 bp
603 amino acids  =  68.4 kDa
Product: β-glucuronidase
baculovirus recombination region (ORF1629)
3512 .. 4869  =  1358 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
3512 .. 4869  =  1358 bp
contains part of ORF1629
AmpR
5891 .. 6751  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5891 .. 6682  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5891 .. 6751  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6683 .. 6751  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5891 .. 6751  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
329 .. 1156  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
329 .. 1156  =  828 bp
contains ORF603 and part of lef2
ori
5132 .. 5720  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5132 .. 5720  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
6883 .. 7338  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
6883 .. 7338  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
p6.9 promoter
2987 .. 3302  =  316 bp
baculovirus late basic protein promoter
p6.9 promoter
2987 .. 3302  =  316 bp
baculovirus late basic protein promoter
AmpR promoter
6752 .. 6856  =  105 bp
AmpR promoter
6752 .. 6856  =  105 bp
polyhedrin promoter
3317 .. 3408  =  92 bp
promoter for the baculovirus polyhedrin gene
polyhedrin promoter
3317 .. 3408  =  92 bp
promoter for the baculovirus polyhedrin gene
MCS
3412 .. 3457  =  46 bp
multiple cloning site
MCS
3412 .. 3457  =  46 bp
multiple cloning site
6xHis
3458 .. 3475  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
3458 .. 3475  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
ORF1629
3528 .. 4869  =  1342 bp
446 amino acids  =  49.2 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
3528 .. 4869  =  1342 bp
446 amino acids  =  49.2 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF603
491 .. 1096  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
491 .. 1096  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
lef2
329 .. 453  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
329 .. 453  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
ORF:  73 .. 453  =  381 bp
ORF:  126 amino acids  =  14.4 kDa
ORF:  1488 .. 1814  =  327 bp
ORF:  108 amino acids  =  12.8 kDa
ORF:  2403 .. 2864  =  462 bp
ORF:  153 amino acids  =  17.5 kDa
ORF:  6021 .. 6287  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  491 .. 1096  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  5891 .. 6751  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  535 .. 765  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
ORF:  3019 .. 3345  =  327 bp
ORF:  108 amino acids  =  12.8 kDa
ORF:  1167 .. 2978  =  1812 bp
ORF:  603 amino acids  =  68.4 kDa
ORF:  3528 .. 4781  =  1254 bp
ORF:  417 amino acids  =  46.0 kDa
Click here to try SnapGene

Download pBACgus-1.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.