Resources
Plasmid Files

pFastBac™HT B

Vector for expressing 6xHis-TEV-tagged proteins in insect cells using the Bac-to-Bac® baculovirus system. For other reading frames, use pFastBac™HT A or pFastBac™HT C.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pFastBacHT B Sequence and MappFastBacHT B.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 NgoMIV (127) AvrII (4497) BclI * (4482) HpaI (4346) MfeI (4333) HindIII (4218) KpnI (4216) Acc65I (4212) SphI (4210) PaeR7I - XhoI (4200) PstI (4197) XbaI (4185) BstBI (4180) NotI (4171) SpeI (4163) SacI (4161) Eco53kI (4159) SalI - SgrDI (4151) StuI (4145) EcoRI (4135) BamHI (4128) NcoI - PluTI (4123) SfoI (4121) NarI (4120) KasI (4119) ATG RsrII (4039) BstZ17I (3884) SnaBI (3881) BbsI (3757) Pc promoter BsrGI (3410) PflFI - Tth111I (3228) BsmBI (3182) BseRI (2961) SacII (2768) BstXI (2717) MscI (2709) NaeI (129) PvuI (1007) FspI (1153) BpmI (1307) AlwNI (1855) BseYI (1960) PspFI (1964) BspQI - SapI (2381) pFastBac™HT B 4857 bp
NgoMIV  (127)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
AvrII  (4497)
1 site
C C T A G G G G A T C C
BclI  (4482)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
HpaI  (4346)
1 site
G T T A A C C A A T T G
MfeI  (4333)
1 site
C A A T T G G T T A A C
HindIII  (4218)
1 site
A A G C T T T T C G A A
KpnI  (4216)
1 site
G G T A C C C C A T G G
Acc65I  (4212)
1 site
G G T A C C C C A T G G
SphI  (4210)
1 site
G C A T G C C G T A C G
PaeR7I  (4200)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (4200)
1 site
C T C G A G G A G C T C
PstI  (4197)
1 site
C T G C A G G A C G T C
XbaI  (4185)
1 site
T C T A G A A G A T C T
BstBI  (4180)
1 site
T T C G A A A A G C T T
NotI  (4171)
1 site
G C G G C C G C C G C C G G C G
SpeI  (4163)
1 site
A C T A G T T G A T C A
SacI  (4161)
1 site
G A G C T C C T C G A G
Eco53kI  (4159)
1 site
G A G C T C C T C G A G
SalI  (4151)
1 site
G T C G A C C A G C T G
SgrDI  (4151)
1 site
C G T C G A C G G C A G C T G C
StuI  (4145)
1 site
A G G C C T T C C G G A
EcoRI  (4135)
1 site
G A A T T C C T T A A G
BamHI  (4128)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
NcoI  (4123)
1 site
C C A T G G G G T A C C
PluTI  (4123)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (4121)
1 site
G G C G C C C C G C G G
NarI  (4120)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (4119)
1 site
G G C G C C C C G C G G
RsrII  (4039)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstZ17I  (3884)
1 site
G T A T A C C A T A T G
SnaBI  (3881)
1 site
T A C G T A A T G C A T
BbsI  (3757)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsrGI  (3410)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PflFI  (3228)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3228)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsmBI  (3182)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BseRI  (2961)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
SacII  (2768)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BstXI  (2717)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
MscI  (2709)
1 site
T G G C C A A C C G G T
NaeI  (129)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
PvuI  (1007)
1 site
C G A T C G G C T A G C
FspI  (1153)
1 site
T G C G C A A C G C G T
BpmI  (1307)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AlwNI  (1855)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BseYI  (1960)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (1964)
1 site
C C C A G C G G G T C G
BspQI  (2381)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2381)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
AmpR
589 .. 1449  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   589 .. 657  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
589 .. 1449  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   658 .. 1449  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
589 .. 1449  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
1620 .. 2208  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1620 .. 2208  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
GmR
2802 .. 3335  =  534 bp
177 amino acids  =  19.4 kDa
Product: gentamycin acetyltransferase
confers resistance to gentamycin
GmR
2802 .. 3335  =  534 bp
177 amino acids  =  19.4 kDa
Product: gentamycin acetyltransferase
confers resistance to gentamycin
f1 ori
2 .. 457  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
2 .. 457  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
Tn7R
2511 .. 2735  =  225 bp
mini-Tn7 element (right end of the Tn7 transposon)
Tn7R
2511 .. 2735  =  225 bp
mini-Tn7 element (right end of the Tn7 transposon)
Tn7L
4510 .. 4675  =  166 bp
mini-Tn7 element (left end of the Tn7 transposon)
Tn7L
4510 .. 4675  =  166 bp
mini-Tn7 element (left end of the Tn7 transposon)
SV40 poly(A) signal
4347 .. 4481  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4347 .. 4481  =  135 bp
SV40 polyadenylation signal
AmpR promoter
484 .. 588  =  105 bp
AmpR promoter
484 .. 588  =  105 bp
MCS
4119 .. 4223  =  105 bp
multiple cloning site
MCS
4119 .. 4223  =  105 bp
multiple cloning site
polyhedrin promoter
3904 .. 3995  =  92 bp
promoter for the baculovirus polyhedrin gene
polyhedrin promoter
3904 .. 3995  =  92 bp
promoter for the baculovirus polyhedrin gene
Pc promoter
3524 .. 3552  =  29 bp
   Segment 3:  -10  
   3524 .. 3529  =  6 bp
class 1 integron promoter
Pc promoter
3524 .. 3552  =  29 bp
   Segment 2:  
   3530 .. 3546  =  17 bp
class 1 integron promoter
Pc promoter
3524 .. 3552  =  29 bp
   Segment 1:  -35  
   3547 .. 3552  =  6 bp
class 1 integron promoter
Pc promoter
3524 .. 3552  =  29 bp
3 segments
class 1 integron promoter
6xHis
4062 .. 4079  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
4062 .. 4079  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
ATG
4050 .. 4052  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
4050 .. 4052  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
TEV Site
4101 .. 4121  =  21 bp
7 amino acids  =  870.0 Da
Product: tobacco etch virus (TEV) protease
recognition and cleavage site
TEV Site
4101 .. 4121  =  21 bp
7 amino acids  =  870.0 Da
Product: tobacco etch virus (TEV) protease
recognition and cleavage site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter