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pFastBac™ Dual

Dual-promoter vector for co-expression of two proteins in insect cells using the Bac-to-Bac® baculovirus system.

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pFastBac Dual.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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AvrII (4978) HpaI (4827) MfeI (4814) HindIII (4699) PstI (4690) XbaI (4678) BstBI (4673) NotI (4664) SpeI (4656) SacI (4654) Eco53kI (4652) SalI - SgrDI (4644) StuI (4638) EcoRI (4628) BssHII (4621) RsrII (4613) BamHI (4606) BstZ17I (4458) PacI (4384) BbsI (4325) SmaI (4321) TspMI - XmaI (4319) PaeR7I - XhoI (4311) NcoI (4305) BmtI (4302) NheI (4298) PvuII (4295) NsiI (4291) SphI (4284) KpnI (4278) Acc65I (4274) SnaBI (3982) BsrGI (3510) PflFI - Tth111I (3328) BsmBI (3282) BseRI (3061) EcoRV (2925) SacII (2868) BstXI (2817) MscI (2809) NgoMIV (227) NaeI (229) BsaHI (936) PvuI (1107) FspI (1253) BpmI (1407) AlwNI (1955) BseYI (2060) PspFI (2064) BspQI - SapI (2481) pFastBac™ Dual 5238 bp
AvrII  (4978)
1 site
C C T A G G G G A T C C
HpaI  (4827)
1 site
G T T A A C C A A T T G
MfeI  (4814)
1 site
C A A T T G G T T A A C
HindIII  (4699)
1 site
A A G C T T T T C G A A
PstI  (4690)
1 site
C T G C A G G A C G T C
XbaI  (4678)
1 site
T C T A G A A G A T C T
BstBI  (4673)
1 site
T T C G A A A A G C T T
NotI  (4664)
1 site
G C G G C C G C C G C C G G C G
SpeI  (4656)
1 site
A C T A G T T G A T C A
SacI  (4654)
1 site
G A G C T C C T C G A G
Eco53kI  (4652)
1 site
G A G C T C C T C G A G
SalI  (4644)
1 site
G T C G A C C A G C T G
SgrDI  (4644)
1 site
C G T C G A C G G C A G C T G C
StuI  (4638)
1 site
A G G C C T T C C G G A
EcoRI  (4628)
1 site
G A A T T C C T T A A G
BssHII  (4621)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (4613)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BamHI  (4606)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BstZ17I  (4458)
1 site
G T A T A C C A T A T G
PacI  (4384)
1 site
T T A A T T A A A A T T A A T T
BbsI  (4325)
1 site
G A<